Characterization of hemolytic and cytotoxic gallysins: A relationship with arylphorins
Characterization of hemolytic and cytotoxic gallysins: A relationship with arylphorins
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DOI：
10.1016/s0145-305x(97)00011-6
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发表时间：
1997-05-01
影响因子：
2.9
通讯作者：
Aston, WP
中科院分区：
文献类型：
--
作者：
Beresford, PJ;BasinskiGray, JM;Aston, WP
Gallysin-1是大蜡螟幼虫保护性反应中的一种可诱导效应蛋白，是从无免疫细胞血淋巴中分离的溶血活性物质（HAM）的75 kDa组分。抗菌原蛋白Gal lysin-1的前20个N-末端氨基酸的序列与Galleria arylphorin Lhp 76（幼虫血淋巴蛋白76）的前20个氨基酸的预测序列相同。针对Gallysin-1的20个氨基酸N-末端肽的鼠单克隆抗体（GYPQYHYDVETRKLDPSLVN）为Gallysin-1和Lhp 76之间的连接提供了额外的证据，并用于进一步表征HAM。HAM最初被表征为两种蛋白质Gallysin-1和69 kDa组分的混合物，现在被鉴定为450-500 kDa的异源多聚体，称为Gallysin。在诱导的免疫应答的效应期期间，胃肠道溶素的体内水平升高。该单克隆抗体抑制Gallysin的溶血活性。除了对哺乳动物红细胞具有溶血活性外，Gallysin还对人肿瘤细胞系K562具有细胞毒性活性。脂多糖（LPS）和铜绿假单胞菌疫苗在保护性应答中诱导细胞毒活性，其达到其最大值。从接种后2小时收集的无细胞血淋巴中获得的部分纯化的细胞毒性物质（Cyt-M）具有溶血活性，并显示出与Gallysin和Lhp 76的结构相似性。先前建立的Gallysin-1作为大蜡螟保护性反应中的效应蛋白的作用表明芳基蛋白可能在昆虫免疫反应中起作用。(C)1997 Elsevier Science Ltd.
Gallysin-1, an inducible effector protein in the protective response of Galleria mellonella larvae is a 75 kDa component of hemolytically active material (HAM) isolated from immune cell-free hemolymph. The sequence of the first 20 N-terminal amino acids of the antibacterial pro rein Gal lysin-l is identical to the predicted sequence of the first 20 amino acids of the Galleria arylphorin Lhp76 (larval hemolymph protein 76). A murine monoclonal antibody to the 20 amino acid N-terminal peptide of Gallysin-1 (GYPQYHYDVETRKLDPSLVN) provides additional evidence for a link between Gallysin-1 and Lhp76, and is used to characterize HAM further. HAM, initially characterized as a mixture of two proteins, Gallysin-1 and a 69 kDa component is now identified as a 450-500 kDa heteromultimer, designated Gallysin. In vivo levels of Gallysin rise during the effector phase of an induced immune response. The monoclonal antibody inhibits the hemolytic activity of Gallysin. In addition to a hemolytic activity for mammalian erythrocytes, Gallysin possesses a cytotoxic activity for the human tumor cell line, K562. Lipopolysaccharides (LPS) and Pseudomonas aeruginosa vaccine induce a cytotoxic activity which reaches its maximum vaccination) in the protective response. The partially purified cytotoxic material (Cyt-M) obtained from cell-free hemolymph collected 2 hours after vaccination has hemolytic activity and shows structural similarities to Gallysin and Lhp76. The previously established role of Gallysin-1 as an effector protein in the protective response of Galleria mellonella indicates that arylphorins may play a role in insect immune responses. (C) 1997 Elsevier Science Ltd.