Identification of NK Cell Receptor Ligands Using a Signaling Reporter System
Identification of NK Cell Receptor Ligands Using a Signaling Reporter System
复制标题
DOI：
10.1007/978-1-60761-362-6_19
复制
发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Iizuka, Koho
中科院分区：
文献类型：
--
作者：
Iizuka, Yoshie-Matsubayashi;Somia, Nikunj V.;Iizuka, Koho
NK细胞应答受抑制和活化信号的平衡调节，反映了靶细胞和效应细胞表面上受体和配体之间相互作用的净效应。孤儿NK细胞受体配体的鉴定是增强我们对NK细胞生物学理解的关键。在这里，我们描述了一种策略（协议）的孤儿NK细胞受体的配体的识别使用信号报告细胞与病毒救援系统相结合。
NK cell responses are regulated by a balance of inhibitory and activating signals, reflecting the net effect of interactions between receptors and ligands on target and effector cell surfaces. The identification of ligands for orphan NK cell receptors is key to enhancing our understanding of NK cell biology. Here we describe a strategy (protocol) for the identification of ligands for orphan NK cell receptors using signaling reporter cells in combination with a virus rescue system.