POU6F1 is the transcription factor that might be involved in cell proliferation of clear cell adenocarcinoma of the ovary
POU6F1 is the transcription factor that might be involved in cell proliferation of clear cell adenocarcinoma of the ovary
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DOI：
10.1111/j.1749-0774.2009.00074.x
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发表时间：
2009-11
期刊：
影响因子：
4.3
通讯作者：
N. Yoshioka;N. Suzuki;A. Uekawa;K. Kiguchi;B. Ishizuka
中科院分区：
文献类型：
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作者：
N. Yoshioka;N. Suzuki;A. Uekawa;K. Kiguchi;B. Ishizuka
卵巢透明细胞腺癌常表现出对抗癌药物的耐药性。我们研究了用于卵巢透明细胞腺癌分子靶向治疗的新分子。对无侵袭潜力的RMG-I细胞和具有侵袭潜力的RMG-V细胞（来源于卵巢透明细胞腺癌）进行互补脱氧核糖核酸微阵列分析。小窝蛋白-1，一种参与细胞运动和侵袭的分子，在两种细胞系之间表现出不同的表达。使用公开的针对小窝蛋白-1的siRNA进行RNA干扰实验。结果显示siRNA抑制了RMG-V细胞的浸润，但癌细胞的增殖也受到抑制。换句话说，RMG-V细胞浸润可能仅仅因为RNA干扰抑制了细胞增殖而被抑制。这些结果表明POU 6 F1可能是一种参与卵巢癌细胞增殖的转录因子。卵巢透明细胞腺癌对标准治疗反应甚微。本研究的结果表明，转录因子POU 6 F1可能是一个新的分子靶点，用于治疗这种癌症。
Clear cell adenocarcinoma of the ovary often shows resistance to anticancer agents. We investigated new molecules to use when developing molecular-targeting therapy for clear cell adenocarcinoma of the ovary. RMG-I cells without invasive potential and RMG-V cells with invasive potential (derived from clear cell adenocarcinoma of the ovary) were subjected to complementary deoxyribonucleic acid microarray analysis. Caveolin-1, a molecule involved in cellular motility and invasion, showed differing expression between the two cell lines. An RNA interference experiment using the published siRNA for caveolin-1 was carried out. The results showed suppression of RMG-V cell infiltration by siRNA, but proliferation of the cancer cells was also suppressed. In other words, RMG-V cell infiltration may have been suppressed simply because cell proliferation was suppressed by RNA interference. These findings suggested that POU6F1 might be a transcription factor involved in the proliferation of ovarian cancer cells. Clear cell adenocarcinoma of the ovary shows little response to standard therapy. The results of the present study suggest that the transcription factor POU6F1 could be a new molecular target for treatment of this cancer.