Determination of dimethyl trisulfide in rabbit blood using stir bar sorptive extraction gas chromatography-mass spectrometry
Determination of dimethyl trisulfide in rabbit blood using stir bar sorptive extraction gas chromatography-mass spectrometry
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DOI：
10.1016/j.chroma.2016.07.046
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发表时间：
2016-08-26
影响因子：
4.1
通讯作者：
Logue, Brian A.
中科院分区：
文献类型：
--
作者：
Manandhar, Erica;Maslamani, Nujud;Logue, Brian A.
意外或故意接触氰化物中毒会造成严重的健康风险。目前食品和药物管理局批准的氰化物中毒解毒剂可能是有效的，但每种解毒剂都存在具体的主要局限性，如有效剂量大、起效延迟或对通常局限于特定器官的酶的依赖。二甲基三硫化物 (DMTS) 是一种硫供体，通过将氰化物转化为硫氰酸盐（一种相对无毒的氰化物代谢物）来解毒，是一种有前途的下一代氰化物解毒剂。尽管目前还没有一种经过验证的分析方法可以从任何基质中分析 DMTS，但一种方法对于批准 DMTS 作为氰化物中毒治疗剂至关重要。因此，开发并验证了搅拌棒吸附萃取 (SBSE) 气相色谱质谱 (GC MS) 方法，用于分析兔全血中的 DMTS。血液酸变性后，将 DMTS 提取到聚二甲基硅氧烷涂层的搅拌棒中。然后将 DMTS 从搅拌棒上热解吸并通过 GC MS 进行分析。使用该方法对DMTS的检测限为0.06μM，动态范围为0.5-100μM。对于质量控制标准，以相对标准偏差百分比测量的精密度低于10％，准确度在标称浓度的15％以内。这里描述的方法将允许进一步研究 DMTS 作为氰化物中毒的有前途的解毒剂。 (c) 2016 Elsevier B.V. 保留所有权利。
Cyanide poisoning by accidental or intentional exposure poses a severe health risk. The current Food and Drug Administration approved antidotes for cyanide poisoning can be effective, but each suffers from specific major limitations concerning large effective dosage, delayed onset of action, or dependence on enzymes generally confined to specific organs. Dimethyl trisulfide (DMTS), a sulfur donor that detoxifies cyanide by converting it into thiocyanate (a relatively nontoxic cyanide metabolite), is a promising next generation cyanide antidote. Although a validated analytical method to analyze DMTS from any matrix is not currently available, one will be vital for the approval of DMTS as a therapeutic agent against cyanide poisoning. Hence, a stir bar sorptive extraction (SBSE) gas chromatography mass spectrometry (GC MS) method was developed and validated for the analysis of DMTS from rabbit whole blood. Following acid denaturation of blood, DMTS was extracted into a polydimethylsiloxane-coated stir bar. The DMTS was then thermally desorbed from the stir bar and analyzed by GC MS. The limit of detection of DMTS using this method was 0.06 mu M with dynamic range from 0.5-100 mu M. For quality control standards, the precision, as measured by percent relative standard deviation, was below 10%, and the accuracy was within 15% of the nominal concentration. The method described here will allow further investigations of DMTS as a promising antidote for cyanide poisoning. (c) 2016 Elsevier B.V. All rights reserved.