Spatio-temporal monitoring of deep-sea communities using metabarcoding of sediment DNA and RNA.
Spatio-temporal monitoring of deep-sea communities using metabarcoding of sediment DNA and RNA.
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作者：
Guardiola M;Wangensteen OS;Taberlet P;Coissac E;Uriz MJ;Turon X
我们评估了时空格局的多样性在深海沉积物群落使用元条码。我们选择了一个最近开发的真核生物标记的基础上的v7区的18 S rRNA基因。我们的研究是在一个海底峡谷及其邻近的斜坡在地中海西北部，采样沿着在两个不同的季节的深度梯度。共发现5,569个分子操作分类单位（MOTU），以后生动物、泡孔动物和根瘤菌为主。在后生动物中，线虫类、节肢动物类和环节动物类是最具多样性的。我们发现了一个显着的异质性在所有尺度上，沉积层之间的重要差异和显着的变化，社区组成与区域（峡谷与斜坡），深度和季节。我们比较了从元巴编码DNA和RNA获得的信息，发现更多的总MOTU和更多的MOTU每个样本与DNA（约。分别增长20%和40%）。这两个数据集显示出总体上相似的空间趋势，但大多数群体具有较高的MOTU丰富度与DNA模板，而其他人，如线虫，在RNA数据集中更多样化。我们提供metabarcoding协议和指导方针，这些关键社区的生物监测，以产生适用于管理工作的信息。
We assessed spatio-temporal patterns of diversity in deep-sea sediment communities using metabarcoding. We chose a recently developed eukaryotic marker based on the v7 region of the 18S rRNA gene. Our study was performed in a submarine canyon and its adjacent slope in the Northwestern Mediterranean Sea, sampled along a depth gradient at two different seasons. We found a total of 5,569 molecular operational taxonomic units (MOTUs), dominated by Metazoa, Alveolata and Rhizaria. Among metazoans, Nematoda, Arthropoda and Annelida were the most diverse. We found a marked heterogeneity at all scales, with important differences between layers of sediment and significant changes in community composition with zone (canyon vs slope), depth, and season. We compared the information obtained from metabarcoding DNA and RNA and found more total MOTUs and more MOTUs per sample with DNA (ca. 20% and 40% increase, respectively). Both datasets showed overall similar spatial trends, but most groups had higher MOTU richness with the DNA template, while others, such as nematodes, were more diverse in the RNA dataset. We provide metabarcoding protocols and guidelines for biomonitoring of these key communities in order to generate information applicable to management efforts.
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影响因子：
3.3
作者：
Brannock, Pamela M.;Wang, Lei;Halanych, Kenneth M.
通讯作者：
Halanych, Kenneth M.
影响因子：
1.6
作者：
Brannock, Pamela M.;Waits, Damien S.;Halanych, Kenneth M.
通讯作者：
Halanych, Kenneth M.
影响因子：
--
作者：
Brustolin, M. C.;Thomas, M. C.;Lana, P. C.
通讯作者：
Lana, P. C.
影响因子：
3.3
作者：
Arroyo AS;López-Escardó D;de Vargas C;Ruiz-Trillo I
通讯作者：
Ruiz-Trillo I
影响因子：
56.9
作者：
Dell'Anno, A;Danovaro, R
通讯作者：
Danovaro, R