Sulfatase-activated fluorophores for rapid discrimination of mycobacterial species and strains
Sulfatase-activated fluorophores for rapid discrimination of mycobacterial species and strains
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DOI：
10.1073/pnas.1222041110
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发表时间：
2013-08-06
影响因子：
11.1
通讯作者：
Bertozzi, Carolyn R.
中科院分区：
文献类型：
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作者：
Beatty, Kimberly E.;Williams, Monique;Bertozzi, Carolyn R.
目前大多数结核病的诊断测试不能揭示引起肺部感染的病原体的种类或菌株，这可能导致不适当的治疗方案和疾病的传播。在这里，我们报告了一种基于遗传保守的分枝杆菌硫酸酯酶的分枝杆菌菌株分配测定法。我们开发了一种硫酸酯酶激活的探针，7-羟基-9H-（1，3-二氯-9，9-二甲基吖啶-2-酮）-硫酸盐，检测天然蛋白质凝胶中的酶活性，允许快速检测分枝杆菌裂解物中的硫酸酯酶。该测定揭示分枝杆菌菌株具有可用于判断种和谱系的独特硫酸酯酶指纹。我们的研究结果表明，潜在的酶激活探针快速病原体歧视传染病。
Most current diagnostic tests for tuberculosis do not reveal the species or strain of pathogen causing pulmonary infection, which can lead to inappropriate treatment regimens and the spread of disease. Here, we report an assay for mycobacterial strain assignment based on genetically conserved mycobacterial sulfatases. We developed a sulfatase-activated probe, 7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one)-sulfate, that detects enzyme activity in native protein gels, allowing the rapid detection of sulfatases in mycobacterial lysates. This assay revealed that mycobacterial strains have distinct sulfatase fingerprints that can be used to judge both the species and lineage. Our results demonstrate the potential of enzyme-activated probes for rapid pathogen discrimination for infectious diseases.