Full-Length Sequences of Two Hepatitis E Virus Isolates Representing an Eastern China-Indigenous Subgroup of Genotype 4
Full-Length Sequences of Two Hepatitis E Virus Isolates Representing an Eastern China-Indigenous Subgroup of Genotype 4
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DOI：
10.1159/000098961
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发表时间：
2007-01
期刊：
影响因子：
4.6
通讯作者：
M. Koike;Kazuaki Takahashi;S. Mishiro;A. Matsui;M. Inao;S. Nagoshi;A. Ohno;S. Mochida;K. Fujiwara
中科院分区：
文献类型：
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作者：
M. Koike;Kazuaki Takahashi;S. Mishiro;A. Matsui;M. Inao;S. Nagoshi;A. Ohno;S. Mochida;K. Fujiwara
虽然大多数戊型肝炎病毒（HEV）感染在日本是“国内”由于本土菌株的存在，仍然有“输入”的情况下，以及。在琦玉医科大学医院收治的83例非甲、非乙、非丙类急性肝病患者中，7例（8.4%）血清HEV-RNA阳性，其中2例有近期中国旅行史，西安1例，上海1例。我们测定了这2例患者的HEV全基因组序列（分离株名称为JKO-ChiSai 98 c和JYI-ChiSai 01 c，均为基因型4），用于系统发育分析。最初，当仅与迄今报道的基因型4的13个全基因组序列进行比较时，我们的2个分离株被认为是新菌株，因为它们显示出与迄今已知序列的显著遗传差异。然而，当我们将最近从中国报道的一组短序列（150 nt）纳入比较时，我们发现我们的2个分离株代表基因型4的一个亚组，这似乎仅限于中国东部。因此，本文报道的2株HEV分离株可作为基因型4 HEV华东地区亚群的全基因组原型。
Although the majority of hepatitis E virus (HEV) infections in Japan are ‘domestic’ due to the presence of indigenous strains, there are still ‘imported’ cases as well. Among 83 patients with non-A, non-B and non-C acute liver diseases admitted to Saitama Medical University Hospital, 7 (8.4%) were positive for serum HEV-RNA, of whom 2 had a recent history of traveling to China, one to Xian and another to Shanghai. We determined the full-genome sequences of HEV from these 2 patients (isolate names are JKO-ChiSai98c and JYI-ChiSai01c, genotype 4 in both) for phylogenetic analyses. Initially, when compared only to the 13 full-genome sequences of genotype 4 so far reported, our 2 isolates were thought to be novel strains because they showed a significant genetic difference from the sequences known to date. However, when we included a set of short sequences (150 nt) recently reported from China in the comparison, we found that our 2 isolates represent a subgroup of genotype 4, which seems to be restricted to eastern China. In conclusion, the 2 HEV isolates reported here could serve as full-genome prototypes for an eastern China-indigenous subgroup of the genotype 4 HEV.