A complex genetic interaction between Arabidopsis thaliana TOC1 and CCA1/LHY in driving the circadian clock and in output regulation
A complex genetic interaction between Arabidopsis thaliana TOC1 and CCA1/LHY in driving the circadian clock and in output regulation
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DOI：
10.1534/genetics.107.072769
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发表时间：
2007-07-01
期刊：
影响因子：
3.3
通讯作者：
Davis, Seth J.
中科院分区：
文献类型：
--
作者：
Ding, Zhaojun;Doyle, Mark R.;Davis, Seth J.
已经提出，昼夜节律钟相关蛋白1（CCA 1）和晚伸长下胚轴（LHY）与CAB表达时间1（TOC 1）一起构成拟南芥昼夜节律钟的中心振荡器。因此，这些基因驱动节奏输出，包括开花和光形态建成的季节控制。为了验证各种生物钟模型，揭示TOC 1和CCA 1/LHY在成花诱导和光形态建成中的遗传关系，我们构建了cca 1 lhytoc 1三突变体和cca 1 toc 1和lhytoc 1双突变体，并测试了各种节律反应和昼夜输出调节。在这里，我们报告说，节奏活动显着衰减cca 1 lhytoc 1。有趣的是，我们还发现，TOC 1调节花的转换在一个CCA 1/LHY依赖的方式，而CCA 1/LHY功能的TOC 1上游的光形态发生过程中的调节。这向地雷影响调查表明，TOC 1和CCA 1/LHY通过不同的策略参与这两个过程。总的来说，我们已经使用遗传学来为先前的建模工作提供直接的实验支持，其中CCA 1/LHY沿着TOC 1驱动昼夜节律振荡器，并且已经表明该时钟对于正确的输出调节是必不可少的。
It has been proposed that CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) together with TIMING OF CAB EXPRESSION 1 (TOC1) make up the central oscillator of the Arabidopsis thaliana circadian clock. These genes thus drive rhythmic outputs, including seasonal control of flowering and photomorphogenesis. To test various clock models and to disclose the genetic relationship between TOC1 and CCA1/LHY in floral induction and photomorphogenesis, we constructed the cca1 lhy toc1 triple mutant and cca1 toc1 and lhy toc1 double mutants and tested various rhythmic responses and circadian output regulation. Here we report that rhythmic activity was dramatically attenuated in cca1 lhy toc1. Interestingly, we also found that TOC1 regulates the floral transition in a CCA1/LHY-dependent manner while CCA1/LHY functions upstream of TOC1 in regulating a photomorphogenic process. This suggests to LIS that TOC1 and CCA1/LHY participate in these two processes through different strategies. Collectively, we have used genetics to provide direct experimental support of previous modeling efforts where CCA1/LHY, along with TOC1, drives the circadian oscillator and have shown that this clock is essential for correct output regulation.