Papain-like cysteine proteases are required for the regulation of photosynthetic gene expression and acclimation to high light stress.
Papain-like cysteine proteases are required for the regulation of photosynthetic gene expression and acclimation to high light stress.
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DOI：
10.1093/jxb/erab101
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发表时间：
2021-04-13
影响因子：
6.9
通讯作者：
Foyer CH
中科院分区：
文献类型：
--
作者：
Alomrani S;Kunert KJ;Foyer CH
叶绿体名义上缺乏半胱氨酸蛋白酶，但在叶绿体或细胞质中表达半胱氨酸蛋白酶抑制剂的拟南芥植物中，叶绿体蛋白质周转和基因表达发生了改变。 叶绿体被认为不含半胱氨酸蛋白酶。利用在叶绿体（PC 系）或细胞质（CYS 系）中表达水稻半胱氨酸蛋白酶抑制剂、米胱抑素 I (OC-I) 的转基因拟南芥系，我们探索了半胱氨酸蛋白酶调节光合作用的假设。 CYS和PC品系比野生型(WT)开花晚，并且开花后积累了更多的生物量。 PC 莲座丛比 WT 积累了更多的叶绿素和类胡萝卜素色素，而 CYS 系的叶色素含量较低。与 WT 相比，光合碳同化作用以及 Rubisco 大亚基蛋白、D1 蛋白和 D1 蛋白磷酸化形式的丰度的强光依赖性降低在 CYS 系中减弱，而在 PC 系中则相反。然而，转基因品系比野生型具有更高含量的 LHC、红细胞、pasbA 和 pasbD 转录物，并且还显示出叶绿体到细胞核信号传导的修饰。我们得出的结论是，半胱氨酸蛋白酶在开花后和响应强光胁迫时加速了叶绿体蛋白质组的重构。半胱氨酸蛋白酶（例如 AtCEP1）的抑制可减缓叶绿体蛋白质降解并刺激光合基因表达和叶绿体到细胞核的信号传导，从而增强抗逆性状。
Chloroplasts nominally lack cysteine proteases but chloroplast protein turnover and gene expression were modified in Arabidopsis plants expressing a cysteine protease inhibitor in the chloroplasts or cytosol. Chloroplasts are considered to be devoid of cysteine proteases. Using transgenic Arabidopsis lines expressing the rice cystatin, oryzacystatin I (OC-I), in the chloroplasts (PC lines) or cytosol (CYS lines), we explored the hypothesis that cysteine proteases regulate photosynthesis. The CYS and PC lines flowered later than the wild type (WT) and accumulated more biomass after flowering. In contrast to the PC rosettes, which accumulated more leaf chlorophyll and carotenoid pigments than the WT, the CYS lines had lower amounts of leaf pigments. High-light-dependent decreases in photosynthetic carbon assimilation and the abundance of the Rubisco large subunit protein, the D1 protein, and the phosphorylated form of D1 proteins were attenuated in the CYS lines and reversed in the PC lines relative to the WT. However, the transgenic lines had higher amounts of LHC, rbcs, pasbA, and pasbD transcripts than the WT, and also showed modified chloroplast to nucleus signalling. We conclude that cysteine proteases accelerate the reconfiguration of the chloroplast proteome after flowering and in response to high-light stress. Inhibition of cysteine proteases, such as AtCEP1, slows chloroplast protein degradation and stimulates photosynthetic gene expression and chloroplast to nucleus signalling, enhancing stress tolerance traits.
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影响因子：
56.9
作者：
Karpinski, S;Reynolds, H;Mullineaux, P
通讯作者：
Mullineaux, P
影响因子：
56.9
作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
6.9
作者：
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通讯作者：
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