In vitro metabolism of plant lignans:: New precursors of mammalian lignans enterolactone and enterodiol
In vitro metabolism of plant lignans:: New precursors of mammalian lignans enterolactone and enterodiol
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DOI：
10.1021/jf010038a
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发表时间：
2001-07-01
影响因子：
6.1
通讯作者：
Adlercreutz, H
中科院分区：
文献类型：
--
作者：
Heinonen, S;Nurmi, T;Adlercreutz, H
研究了植物木脂素matairesinol、开环异落叶松树脂醇、松脂醇、阿姜树脂醇、牛蒡苷元、7-羟基matairesinol、异落叶松树脂醇和落叶松树脂醇通过人粪便微生物群落的代谢，以研究它们作为哺乳动物木脂素前体的性质。采用高效液相色谱-库仑电极阵列检测器对木脂素前体及哺乳动物木脂素肠内酯和肠二醇进行定量分析。代谢产物，包括哺乳动物木脂素，其特征在于作为三甲基甲硅烷基衍生物的气相色谱-质谱。将马塔树脂酚、开环异落叶松树脂酚、落叶松树脂酚和松松脂醇仅转化为哺乳动物木脂素。除了哺乳动物木脂素外，还分离并初步鉴定了几种代谢物，如双辛树脂醇和牛蒡苷元。通过与真实对照品的比较，7-羟基马台树脂酚的代谢产物被表征为肠内酯和7-羟基肠内酯。一个代谢计划，描述了最丰富的新的哺乳动物木脂素前体，松油醇和lariciresinol的转换。
The metabolism of the plant lignans matairesinol, secoisolariciresinol, pinoresinol, syringaresinol, arctigenin, 7-hydroxymatairesinol, isolariciresinol, and lariciresinol by human fecal microflora was investigated to study their properties as mammalian lignan precursors. The quantitative analyses of lignan precursors and the mammalian lignans enterolactone and enterodiol were performed by HPLC with coulometric electrode array detector. The metabolic products, including mammalian lignans, were characterized as trimethylsilyl derivatives by gas chromatography-mass spectrometry. Matairesinol, secoisolariciresinol, lariciresinol, and pinoresinol were converted to mammalian lignans only. Several metabolites were isolated and tentatively identified as for syringaresinol and arctigenin in addition to the mammalian lignans. Metabolites of 7-hydroxymatairesinol were characterized as enterolactone and 7-hydroxyenterolactone by comparison with authentic reference compounds. A metabolic scheme describing the conversion of the most abundant new mammalian lignan precursors, pinoresinol and lariciresinol, is presented.