Expression of apolipoprotein E in normal and diverse neurodegenerative disease brain
Expression of apolipoprotein E in normal and diverse neurodegenerative disease brain
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DOI：
10.1097/00001756-199607290-00008
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发表时间：
1996-07-29
期刊：
影响因子：
1.7
通讯作者：
Sasaki, H
中科院分区：
文献类型：
--
作者：
Bao, F;Arai, H;Sasaki, H
采用免疫组化和免疫印迹法对21例阿尔茨海默病（AD）、9例弥漫性路易体病（DLBD）、6例进行性核上性麻痹（PSP）和5例帕金森病（PD）患者以及20例正常人的脑组织进行了载脂蛋白E （ApoE）检测。与中性缓冲福尔马林、70%乙醇或微波变性的组织相比，bouin固定的组织保存ApoE抗原性最佳。ApoE免疫反应性在老年斑、神经元内和神经元外缠结以及多种神经元及其过程和星形胶质细胞中表现突出。值得注意的是，PD和DLBD的PSP和路易小体中的缠结均缺乏ApoE免疫反应性。大脑皮层免疫印迹显示与mel有免疫反应的ApoE带。wt为34 kDa。我们的研究结果表明ApoE并不是DLBD、PSP和PD中神经元包涵体发育的关键因素。
BRAINS from 21 patients with Alzheimer's disease (AD), nine with diffuse Lewy body disease (DLBD), six with progressive supranuclear palsy (PSP) and five with Parkinson's disease (PD) as well as 20 normal subjects were examined to detect apolipoprotein E (ApoE) by immunohistochemistry and immunoblotting. ApoE antigenicity was optimally preserved in Bouin-fixed tissues compared with those fixed in neutral-buffered formalin, 70% ethanol or denatured by microwave energy. ApoE immunoreactivity was prominent in senile plaques and in intra- and extra-neuronal tangles, as well as in a diverse neurones and their processes and astroglial cells. Notably, tangles in PSP and Lewy bodies in PD and DLBD were both devoid of ApoE immunoreactivity. Western blots of cerebral cortex revealed an immunoreactive ApoE band with mel. wt of 34 kDa. Our results suggest that ApoE is not a crucial factor in the development of neuronal inclusions in DLBD, PSP and PD.