Erythropoietin alone induces erythroid burst formation by human embryonic but not adult BFU-E in unicellular serum-free culture.
Erythropoietin alone induces erythroid burst formation by human embryonic but not adult BFU-E in unicellular serum-free culture.
复制标题
在单细胞无血清培养物中，促红细胞生成素单独诱导人胚胎 BFU-E 形成红细胞爆发，但不能诱导成人 BFU-E 形成。
作者：
M. Valtieri;M. Gabbianelli;E. Pelosi;E. Bassano;S. Petti;G. Russo;Ugo Testa;Cesare Peschle
来自成人外周血的红系祖细胞（BFU-E）在补充有至少两种生长因子，即促红细胞生成素（Ep）和白细胞介素-3（IL-3）或粒细胞-巨噬细胞集落刺激因子（GM-CSF）的半固体培养物中产生红系爆发。我们分析了人胚胎BFU-E与成人外周血祖细胞相比的促红细胞生成素需求：这基本上是在用人重组造血素处理的部分或高度纯化的祖细胞的无胎牛血清（FCS-）甲基纤维素培养物中进行评价的。在低接种浓度（2 × 10（3）细胞/皿）下，仅用Ep处理时，纯化的胚胎BFU-E产生红系爆发：进一步加入IL-3或GM-CSF对BFU-E克隆效率没有影响，尽管爆发的大小以剂量依赖性方式增加，特别是IL-3。在相似的接种浓度（即，10（3）个细胞/培养皿）下，纯化的成体BFU-E在Ep和GM-CSF或IL-3存在下有效地产生红系爆发，而仅在Ep存在下仅观察到少数小的红系集落。在最后一系列的实验中，纯化的胚胎BFU-E的单细胞FCS培养物在Ep单独存在下引起红系爆发。此外，通过进一步添加GM-CSF或IL-3，Ep诱导的克隆效率未被改变。单细胞FCS-高度纯化的成人外周血祖细胞的培养物在Ep单独存在下不产生红系爆发。需要添加GM-CSF或IL-3以产生BFU-E集落。这些研究表明，在人胚胎期，BFU-E只需要Ep就能有效地形成红细胞系爆发，而IL-3和GM-CSF基本上能增强早期红细胞生成前体的增殖。
Erythroid progenitors (BFU-E) from adult human peripheral blood generate erythroid bursts in semisolid culture supplemented with at least two growth factors, ie, erythropoietin (Ep) and interleukin-3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF). We have analyzed the hematopoietin(s) requirement of human embryonic BFU-E, as compared to that of adult peripheral blood progenitors: This was basically evaluated in fetal calf serum-free (FCS-) methylcellulose culture of partially or highly purified progenitors treated with human recombinant hemopoietins. At a low seeding concentration (2 x 10(3) cells/dish) purified embryonic BFU-E generated erythroid bursts when treated only with Ep: Further addition of IL-3 or GM-CSF had no effect on BFU-E cloning efficiency, although the size of bursts was increased in a dose-dependent manner, particularly with IL-3. At a similar seeding concentration (ie, 10(3) cells/dish), purified adult BFU-E efficiently generated erythroid bursts in the presence of Ep and GM-CSF or IL-3, while only few small erythroid colonies were observed in the presence of Ep alone. In a final series of experiments, unicellular FCS-cultures of purified embryonic BFU-E gave rise to erythroid bursts in the presence of Ep alone. Furthermore, the cloning efficiency induced by Ep was unmodified by further addition of GM-CSF or IL-3. Unicellular FCS- cultures of highly purified adult peripheral blood progenitors generated no erythroid bursts in the presence of Ep alone. The addition of GM-CSF or IL-3 was required to generate BFU-E colonies. These studies indicate that in human embryonic life, BFU-E require only Ep for efficient erythroid burst formation, while IL-3 and GM-CSF essentially enhance the proliferation of early erythropoietic precursors.
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影响因子：
4.4
作者：
M. Valtieri;D. Tweardy;D. Caracciolo;Keith R. Johnson;F. Mavilio;S. Altmann;D. Santoli;G. Rovera
通讯作者：
M. Valtieri;D. Tweardy;D. Caracciolo;Keith R. Johnson;F. Mavilio;S. Altmann;D. Santoli;G. Rovera
DOI：
10.1073/pnas.84.24.9035
发表时间：
1987-12-01
影响因子：
11.1
作者：
IKEBUCHI, K;WONG, GG;OGAWA, M
通讯作者：
OGAWA, M
L-亮氨酸甲酯调节人自然杀伤细胞功能：人外周血单核细胞的单核细胞依赖性消耗。
DOI：
--
发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Thiele,DL;Lipsky,PE
通讯作者：
Lipsky,PE
γ-干扰素改变新生儿和成人红细胞中的珠蛋白基因表达。
影响因子：
20.3
作者：
Miller,BA;Perrine,SP;Antognetti,G;Perlmutter,DH;Emerson,SG;Sieff,C;Faller,DV
通讯作者：
Faller,DV
重组长臂猿白细胞介素 3 支持培养中人多谱系集落和母细胞集落的形成：与重组人粒细胞-巨噬细胞集落刺激因子的比较。
影响因子：
20.3
作者：
Leary,AG;Yang,YC;Clark,SC;Gasson,JC;Golde,DW;Ogawa,M
通讯作者：
Ogawa,M