DIGE analysis of rat skeletal muscle proteins using nonionic detergent phase extraction of young adult versus aged gastrocnemius tissue
DIGE analysis of rat skeletal muscle proteins using nonionic detergent phase extraction of young adult versus aged gastrocnemius tissue
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DOI：
10.1016/j.jprot.2010.01.014
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发表时间：
2010-06-16
影响因子：
3.3
通讯作者：
Ohlendieck, Kay
中科院分区：
文献类型：
--
作者：
Donoghue, Pamela;Staunton, Lisa;Ohlendieck, Kay
收缩无力和肌肉质量的丧失是哺乳动物衰老过程的重要特征。与年龄相关的纤维消耗对肌肉新陈代谢、纤维类型分布和神经肌肉系统的整体生理完整性有深远的影响。本研究使用基于质谱学的蛋白质组学来研究衰老的大鼠肌肉蛋白质组的命运。利用非离子洗涤剂相提取，本报告显示，衰老的腓肠肌在洗涤剂提取部分和衰老肌肉组织的水蛋白补体中都表现出普遍扰动的蛋白质表达模式。在洗涤剂提取物中，ATP合成酶、异柠檬酸脱氢酶、烯醇化酶、原肌球蛋白和β-肌动蛋白的表达增加。不同亚型的肌酸激酶和禁忌素表现出不同的变化。在水相组分中，苹果酸脱氢酶、磺基转移酶、磷酸丙糖异构酶、醛缩酶、Cofilin-2和乳酸脱氢酶水平升高。有趣的是，相同蛋白质种类的不同的二维斑点对铜/锌超氧化物歧化酶、白蛋白、膜联蛋白A4和磷酸乙醇酸磷酸酶的影响是不同的。线粒体Hsp60、Hsp71和核苷二磷酸激酶B在衰老肌肉中的丰度降低。大多数改变的蛋白质被发现参与线粒体代谢、糖酵解、代谢运输、调节过程、细胞应激反应、解毒机制和肌肉收缩。(C)2010爱思唯尔B.V.保留所有权利。
Contractile weakness and loss of muscle mass are critical features of the aging process in mammalians. Age-related fibre wasting has a profound effect on muscle metabolism, fibre type distribution and the overall physiological integrity of the neuromuscular system. This study has used mass spectrometry-based proteomics to investigate the fate of the aging rat muscle proteome. Using nonionic detergent phase extraction, this report shows that the aged gastrocnemius muscle exhibits a generally perturbed protein expression pattern in both the detergent-extracted fraction and the aqueous protein complement from senescent muscle tissue. In the detergent-extracted fraction, the expression of ATP synthase, isocitrate dehydrogenase, enolase, tropomyosin and beta-actin was increased. Different isoforms of creatine kinase and prohibitin showed differential changes. In the aqueous fraction, malate dehydrogenase, sulfotransferase, triosephosphate isomerase, aldolase, cofilin-2 and lactate dehydrogenase showed increased levels. Interestingly, differential effects on dissimilar 2-D spots of the same protein species were shown for Cu/Zn superoxide dismutase, albumin, annexin A4 and phosphoglycolate phosphatase. Mitochondrial Hsp60, Hsp71 and nucleoside diphosphate kinase B exhibited a reduced abundance in aged muscle. The majority of altered proteins were found to be involved in mitochondrial metabolism, glycolysis, metabolic transportation, regulatory processes, the cellular stress response, detoxification mechanisms and muscle contraction. (C) 2010 Elsevier B.V. All rights reserved.