[Susceptibility of methicillin-resistant Staphylococcus aureus to various antibiotics. Classification by aminoglycoside-modifying enzymes and antibiotics active against MRSA].
[Susceptibility of methicillin-resistant Staphylococcus aureus to various antibiotics. Classification by aminoglycoside-modifying enzymes and antibiotics active against MRSA].
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耐甲氧西林金黄色葡萄球菌对多种抗生素的敏感性。
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
T. Takeuchi
中科院分区：
文献类型：
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作者：
S. Kondo;Y. Ikeda;S. Hattori;M. Hamada;T. Takeuchi
对1986年至1990年在大阪某医院分离的50株耐甲氧西林金黄色葡萄球菌（MRSA）进行了14种氨基糖苷类抗生素（包括临床使用的10种抗生素）的mic测定。阿贝卡星（ABK）在≤0.20 ~ 6.25微克/ml的浓度下对所有菌株的生长均有抑制作用，抑制作用最强。链霉素对除1株抗性菌株（大于100微克/ml）外的所有菌株均有良好的活性（1.56 ~ 6.25微克/ml）。根据对卡那霉素（98%耐药）、妥布霉素（84%耐药）、庆大霉素（62%耐药）、阿米卡星（36%耐药）和ABK（0%耐药）的敏感性将MRSA菌株分为5类；0型不产生氨基糖苷修饰酶，1a型产生APH（3′），1b型产生AAD（4′，4′），2a型产生APH（2′）/AAC（6′）和APH（3′），2b型产生APH（2′）/AAC（6′）和AAD（4′，4′）。随机筛选发现除氨基糖苷类抗生素外，万古霉素（VCM）等13种已知抗生素对MRSA有活性。其中，大霉素（0.013 ~ 0.050微克/ml）的活性最强，灰黄素A和灰黄素B（0.10 ~ 0.39微克/ml）和马卡霉素（0.05 ~ 0.20微克/ml）的活性较VCM（0.39 ~ 1.56微克/ml）强。新生物素（小于或等于0.20 ~ 0.78微克/ml）也具有良好的活性。
MICs of 14 aminoglycoside antibiotics including 10 of those used clinically were determined against 50 strains of methicillin-resistant Staphylococcus aureus (MRSA) which had been isolated at a hospital in Osaka between 1986 and 1990. Arbekacin (ABK) inhibited the growth of all strains at less than or equal to 0.20-6.25 micrograms/ml, showing the most potent activities. Streptomycin showed good activities (1.56-6.25 micrograms/ml) against all strains except one resistant strain (greater than 100 micrograms/ml). Based on susceptibilities to kanamycin (98% resistant), tobramycin (84%), gentamicin (62%), amikacin (36%) and ABK (0%), MRSA strains were classified into 5 types; type 0 producing no aminoglycoside-modifying enzyme, type 1a producing APH(3'), type 1b producing AAD(4', 4''), type 2a producing APH (2'')/AAC(6') and APH(3'), and type 2b producing APH(2'')/AAC(6') and AAD(4', 4''). In addition to the aminoglycoside antibiotics, 13 known antibiotics including vancomycin (VCM) were found active against MRSA upon random screening. Taitomycin (0.013-0.050 micrograms/ml) was the most potent, and griseoluteins A and B (each 0.10-0.39 micrograms/ml), and macarbomycin (0.05-0.20 micrograms/ml) were more active than VCM (0.39-1.56 micrograms/ml). Novobiocin (less than or equal to 0.20-0.78 micrograms/ml) also showed good activities.