Basolateral amygdala efferents to the ventral subiculum preferentially innervate pyramidal cell dendritic spines
Basolateral amygdala efferents to the ventral subiculum preferentially innervate pyramidal cell dendritic spines
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DOI：
10.1016/s0006-8993(03)03017-8
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发表时间：
2003-08-15
期刊：
影响因子：
2.9
通讯作者：
Totterdell, S
中科院分区：
文献类型：
--
作者：
French, SJ;Hailstone, JC;Totterdell, S
杏仁核基底外侧和海马体腹侧下带是大脑中两个主要的边缘相关区域，它们都对伏隔核有很大的投射。伏隔核被认为是边缘-运动的接口，因为这些边缘传入和它的躯体运动和自主传出连接。这些传入输入被认为是在伏隔核内的单突触细胞上汇聚，并被假设在情感动机行为等功能中发挥作用。来自基底外侧杏仁核和海马体的输入在伏隔水平的收敛可以用电生理记录方法证明，但这些并不能最终排除多突触机制。事实上，在基底外侧杏仁核和海马体之间存在一个强大的相互投射，其突触细节尚未得到充分研究。我们检查了从杏仁核基底外侧到耻骨下的投射神经元，棘锥体神经元的突触输入。我们用少量生物素化右旋糖酐胺对杏仁核基底外侧的传入事件进行标记，并在枕下显示出顺行标记的纤维。电镜观察标记的杏仁核基底外侧纤维突触后靶结构。通过这项技术，我们已经从解剖学上证明了基底外侧杏仁核优先支配棘下神经元，假定是锥体投影神经元，尽管一些树突和可能的局部回路神经元可能是目标。(C) 2003 Elsevier B.V.版权所有
The basolateral amygdala and the ventral subiculum of the hippocampal formation are two of the major limbic-related regions within the brain, both of which project heavily to the nucleus accumbens. The nucleus accumbens is regarded as the limbic-motor interface, in view of these limbic afferent and its somatomotor and autonomic efferent connections. These afferent inputs have been suggested to converge monosynaptically on cells within the accumbens and are hypothesised to play a role in functions such as affective motivational behaviour. Convergence between inputs from the basolateral amygdala and the hippocampus at the level of the accumbens can be demonstrated with electrophysiological recording methods, but these do not conclusively preclude polysynaptic mechanisms. In fact there is a robust reciprocal projection between the basolateral amygdala and the hippocampus, synaptic details of which have not been fully investigated. We examined the synaptic input from the basolateral amygdala to the projection neurons of the subiculum, the spiny pyramidal neurons. We labelled the afferents from basolateral amygdala with a small injection of biotinylated dextran amine, and revealed the anterogradely labeled fibers within the subiculum. The labeled basolateral amygdala fibers were studied with electron microscopy to identify their postsynaptic target structures. With this technique we have demonstrated anatomically that the basolateral amygdala preferentially innervates spiny subiculum neurons, presumed pyramidal projection neurons, although some dendrites and possibly local circuit neurons may be targeted. (C) 2003 Elsevier B.V. All rights reserved.