Cytotoxicity of Luffa cylindrica (L.) M.Roem. extract against circulating cancer stem cells in hepatocellular carcinoma
Cytotoxicity of Luffa cylindrica (L.) M.Roem. extract against circulating cancer stem cells in hepatocellular carcinoma
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DOI：
10.1016/j.jep.2018.09.034
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发表时间：
2019-01-30
影响因子：
5.4
通讯作者：
Ashour, Mohamed
中科院分区：
文献类型：
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作者：
Abdel-Salam, Ibrahim M.;Awadein, Nihal El-Sayed;Ashour, Mohamed
民族药理学相关性：丝瓜（L）M. Roem。已被认为是治疗各种疾病如炎症性疾病、腹泻和病毒感染的传统药物。通常使用的部分包括水果，种子和leafs.Aim的研究：使用循环肿瘤细胞和癌干细胞分离的外周血中的肝细胞癌patients.Materials和方法：七十五名成年患者报告为第二和第三阶段肝细胞癌，以评估热水提取物的抗癌活性的整个植物的丝瓜cylindrica。抽取血样，从全血中分离循环肿瘤细胞。采用流式细胞术检测并分离肿瘤细胞和肿瘤干细胞。结果：丝瓜热水提取物对肝癌循环肿瘤细胞有明显的杀伤作用，尤其是对CD 133/CD 44（+）细胞亚群有明显的杀伤作用，而对CD 133 */CD 44（+）细胞亚群的杀伤作用较小。丝瓜全草热水提取物可降低肝癌患者血液中癌干细胞的比例，可能用于减少肝癌患者的复发和转移。
Ethnopharmacological relevance: Luffa cylindrica (L) M.Roem. has been recognized as traditional medicine for the treatment of various diseases like inflammatory diseases, diarrhea and viral infections. The usual parts used include fruit, seeds and leaves.Aim of the study: To evaluate the anticancer activity of the hot water extract of the whole plant of Luffa cylindrica using circulating tumor cells and cancer stem cells isolated from the peripheral blood of hepatocellular carcinoma patients in vitro.Materials and methods: Seventy five adult patients who reported as stage II and III Hepatocellular carcinoma were selected. Blood samples were withdrawn and the circulating tumor cells were isolated from the whole blood. Tumor cells and cancer stem cells were detected and isolated by flow cytometric techniques. The isolated cell types were cultured and propagated in the tissue culture facility, the extract was tested on the isolated cells.Results: Luffa cylindrica hot water extract has shown cytotoxic activity against circulating tumor cells of hepatocellular carcinoma especially the cells sub-population CD133/CD44 with little effect among CD133*/ CD44(+) sub-population.Conclusion: Hot water extract of Luffa cylindrica whole plant could decrease the ratio of cancer stem cells in blood of HCC patients and may be used to minimize recurrence and metastasis in hepatocellular carcinoma patients.