EVOLUTIONARY RELATIONSHIPS IN THE GENUS BORDETELLA
EVOLUTIONARY RELATIONSHIPS IN THE GENUS BORDETELLA
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DOI：
10.1111/j.1365-2958.1987.tb01936.x
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发表时间：
1987-11-01
影响因子：
3.6
通讯作者：
RAPPUOLI, R
中科院分区：
文献类型：
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作者：
ARICO, B;GROSS, R;RAPPUOLI, R
百日咳博德特氏菌、副百日咳博德特氏菌和支气管败血性博德特氏菌的百日咳毒素操纵子的核苷酸序列表明，后两个物种包含许多共同的突变，并且可能源自共同的祖先（Arico和Rappuoli，1987）。为了进一步阐明博德特氏菌属物种之间的进化关系，我们克隆并测序了来自additionalB.的启动子区域和编码百日咳毒素S1亚基的基因。百日咳菌株，例如典型菌株 BP 18323 和最近的两种临床分离菌株，即来自瑞典的菌株 BP 13456 和来自意大利的菌株 BP SA1。虽然菌株 BP SA1 和 BP 13456 与已发表的 B.百日咳序列仅由一个碱基对组成，典型菌株 BP 18323 总共包含 11 个碱基对替换。值得注意的是，BP 18323 中发现的 11 个取代中有 9 个也与 B 相同。副百日咳 B. bronchiseptica，强烈表明该菌株与B. bronchiseptica 源自同一祖先。副百日咳 B. bronchiseptica. 序列数据的计算机分析允许构建进化“树”，表明 B.百日咳菌株非常同质，并且与 B. 显着遥远。副百日咳 B. bronchiseptica。因此建议从B转换。副百日咳 B.百日咳似乎不太可能。
The nucleotide sequence of the pertussis toxin operon ofBordetella pertussis, Bordetella parapertussisandBordetella bronchiseptica, has shown that the last two species contain many common mutations and are likely to derive from a common ancestor (Arico and Rappuoli, 1987). To elucidate further the evolutionary relationships between theBordetellaspecies, we have cloned and sequenced the promoter region and the gene coding for the S1 subunit of pertussis toxin from additionalB. pertussisstrains, such as the type strain BP 18323 and two recent clinical isolates, namely strain BP 13456 from Sweden and strain BP SA1 from Italy. While the strains BP SA1 and BP 13456 are shown to differ from the publishedB. pertussissequences by only one base pair, the type strain BP 18323 contains a total of 11 base‐pair substitutions. Remarkably, 9 of the 11 substitutions found in BP 18323 are also common toB. parapertussisandB. bronchiseptica, strongly suggesting that this strain derives from the same ancestor asB. parapertussisandB. bronchiseptica.Computer analysis of the sequence data allows the construction of an evolutionary ‘tree’ showing that theB. pertussisstrains are very homogeneous and significantly distant fromB. parapertussisandB. bronchiseptica.Therefore the proposed conversion fromB. parapertussistoB. pertussisappears highly improbable.