Effect of an Activated Platelet Concentrate on Differentiated Cells Involved in Tissue Healing
Effect of an Activated Platelet Concentrate on Differentiated Cells Involved in Tissue Healing
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DOI：
10.1097/scs.0000000000002540
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发表时间：
2016-05-01
影响因子：
0.9
通讯作者：
Del Fabbro, Massimo
中科院分区：
文献类型：
--
作者：
Brini, Anna T.;Ceci, Caterina;Del Fabbro, Massimo
组织愈合是一个复杂的过程，涉及多种因素，例如激活后血小板释放的细胞和生长因子。如今，浓缩血小板 (PC) 被用于许多不同的医学领域，包括口腔、骨科和重建手术，因为它们可以将生长因子输送到受伤部位，从而增强组织再生。这项体外研究的目的是评估使用手动方案获得的活化血小板浓缩物的无细胞血浆对参与组织愈合的分化细胞的增殖和生物活性的影响。人成骨细胞和真皮成纤维细胞在补充有来自多个供体的 PC 的无血清培养基中生长。 7天后通过MTT测试评估人成骨细胞和人真皮成纤维细胞增殖，并对培养12天的细胞进行计数。培养7天和14天后通过碱性磷酸酶测定测试人成骨细胞的骨分化。相对于在标准条件下生长的细胞而言，向培养基中添加PC导致增殖增加。本研究的结果表明，PC 支持参与伤口愈合和组织再生的终末分化细胞的增殖，证实了其在再生治疗中的有益临床应用。
Tissue healing is a complex process involving several players such as cells and growth factors released from platelets upon activation. Today, platelet concentrates (PCs) are used in many different medical fields including oral, orthopaedic, and reconstructive surgery since they allow growth factors delivery to the injured site, aiming at enhancing tissue regeneration. The purpose of this in vitro study was to evaluate the effect of the acellular plasma of an activated platelet concentrate obtained using a manual protocol, on the proliferation, and biological activity of differentiated cells involved in tissue healing. Human osteoblasts and dermal fibroblasts were grown in serum-free medium supplemented with PC derived from several donors. Human osteoblast and human dermal fibroblast proliferation was assessed by MTT test after 7 days and cells were count up to 12-day incubation. Human osteoblast osteo-differentiation was tested after 7 and 14-day incubation by alkaline phosphatase assay. The addition of PC to the culture medium caused an increased proliferation with respect to cells grown in standard condition. The results of the present study suggest that PC supports the proliferation of terminally differentiated cells involved in wound healing and tissue regeneration, confirming its beneficial clinical application in regenerative therapies.