Cellular expression of a monocarboxylate transporter (MCT1) in the mammary gland and sebaceous gland of mice
Cellular expression of a monocarboxylate transporter (MCT1) in the mammary gland and sebaceous gland of mice
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DOI：
10.1007/s00418-008-0543-3
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发表时间：
2009-03
影响因子：
2.3
通讯作者：
Kumiko Takebe;J. Nio-Kobayashi;H. Takahashi-Iwanaga;T. Yajima;T. Iwanaga
中科院分区：
文献类型：
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作者：
Kumiko Takebe;J. Nio-Kobayashi;H. Takahashi-Iwanaga;T. Yajima;T. Iwanaga
质子偶联单羧酸转运体(MCTs)是乳酸、酮体和其他单羧酸通过质膜转运所必需的，但其转运的方向和底物尚不清楚。本研究检测了MCTs在造脂器官中的表达和亚细胞定位。MCT主要亚型的原位杂交研究发现，MCT1mRNA在乳腺、哈德氏腺和皮脂腺中有强烈表达。MCT1免疫反应主要见于乳腺和哈德氏腺的腺泡细胞内。皮肤、眼皮和阴茎中皮脂腺的肺泡细胞沿整个细胞表面的膜相关的MCT1免疫反应阳性。这些表达MCT1的外分泌腺比具有代表性的脂肪生成器官(如肝脏)拥有更丰富的乙酰辅酶A羧基酶-1的转录，乙酰辅酶A羧化酶-1是脂肪生成的关键酶。由于这些腺体的分泌物含有脂肪作为主要产物，MCT1的细胞定位表明转运蛋白参与了乳酸盐、醋酸盐和其他单羧酸盐的摄取，以产生中链和长链脂肪酸。
Proton-coupled monocarboxylate transporters (MCTs) are essential for the transport of lactate, ketone bodies, and other monocarboxylates through the plasma membrane, but the direction and substrates of transporting in loco remain unclear. The present study examined the expression and subcellular localization of MCTs in lipogenic organs. An in situ hybridization survey of major MCT subtypes detected an intense expression of MCT1 mRNA in the mammary gland, Harderian gland, and sebaceous gland. The MCT1 immunoreactivity was found baso-laterally in acinar cells of the mammary and Harderian glands. Alveolar cells of sebaceous glands in the skin, eyelids, and penis contained the membrane-associated MCT1 immunoreactivity along the entire length of the cell surface at the margin of alveoli. These MCT1-expressing exocrine glands possessed more abundant transcripts of acetyl-CoA carboxylase-1, a key enzyme for lipogenesis, than did representative lipogenetic organs such as the liver. Since the secretions from these glands contain fat as a major product, the cellular localization of MCT1 suggests the involvement of the transporter in the uptake of lactate, acetate, and other monocarboxylates for production of medium- and long-chain fatty acids.