Silencing gene expression of the ethylene-forming enzyme results in a reversible inhibition of ovule development in transgenic tobacco plants
Silencing gene expression of the ethylene-forming enzyme results in a reversible inhibition of ovule development in transgenic tobacco plants
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DOI：
10.2307/3870798
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发表时间：
1999-06-01
期刊：
影响因子：
11.6
通讯作者：
Mariani, C
中科院分区：
文献类型：
--
作者：
De Martinis, D;Mariani, C
为了研究乙烯在植物繁殖中的作用，我们构建了一种具有雌蕊特异性的编码乙烯形成酶1-氨基环丙烷-1-羧酸氧化酶基因的转基因烟草植株。转基因植株的花由于胚珠发育受阻而表现出雌性不育。大孢子未发生，胚珠未成熟。授粉后，花粉管能到达子房，但不能进入未成熟的胚珠。乙烯源处理导致胚珠发育功能恢复，并恢复了花粉管尖端进入胚珠珠孔的引导，从而导致了种子的结实率。如果存在乙烯作用抑制剂，则回收率将被取消。这些结果表明，植物激素乙烯在雌性孢子发生的早期阶段是必需的，并最终使受精成为可能。
To study the role of ethylene in plant reproduction, we constructed transgenic tobacco plants in which the expression of a pistil-specific gene coding for the ethylene-forming enzyme 1-aminocyclopropane-1-carboxylate oxidase was inhibited. Flowers from transgenic plants showed female sterility due to an arrest in ovule development. Megasporogenesis did not occur, and ovules did not reach maturity. When pollinated, pollen tubes were able to reach the ovary but did not penetrate into the immature ovule in transgenic plants. Flower treatment with an ethylene source resulted in a functional recovery of ovule development and restored guidance of the pollen tube tip into the ovule micropyle that resulted in seed set. The recovery was abolished if inhibitors of ethylene action were present. These results demonstrate that the plant hormone ethylene is required during the very early stages of female sporogenesis and ultimately to enable fertilization.