Detection and measurement of microparticles: An evolving research tool for vascular biology
Detection and measurement of microparticles: An evolving research tool for vascular biology
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DOI：
10.1055/s-2007-1000369
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发表时间：
2007-11-01
影响因子：
5.7
通讯作者：
Seldon, Michael
中科院分区：
文献类型：
--
作者：
Enjeti, Anoop K.;Lincz, Lisa F.;Seldon, Michael
微粒是由不同来源的细胞产生的小的膜结合囊泡。现在看来，所有的循环血细胞以及内皮细胞都释放出大小约为1 μ m或更小的膜碎片，这些碎片至少具有母细胞的某些特征。在心血管状态、血栓形成病症和癌症中已经描述了微粒水平升高。检测微粒的各种方法包括流式细胞术、酶联免疫测定和功能测定。流式细胞术具有若干优点，包括其定量和鉴定不同细胞来源的微粒的能力。然而，用于微粒计数的分析前和分析变量的标准化仍然是一个重大挑战。更新的方法正在研究中，一个国际合作组织正在致力于通过流式细胞术对微粒进行检测和定量的标准化。虽然它已经发展成为一个重要的血管生物学研究工具，微粒检测需要进一步的评估和完善，才能成为真正有用的临床工具。
Microparticles are small, membrane-bound vesicles that are generated from cells of different origin. It now appears that all circulating blood cells as well as endothelial cells release membranous fragments similar to 1 mu m in size or smaller bearing at least some characteristics of the parent cell. Elevated levels of microparticles have been described in cardiovascular states, thrombotic conditions, and cancer. Various methods of detection for microparticles include flow cytometry, enzyme-linked immunoassays, and functional assays. Flow cytometry has several advantages including its ability to quantitate and identify microparticles of different cellular origin. However, the standardization of preanalytical and analytical variables for enumeration of microparticles remains a significant challenge. Newer approaches are being investigated, and an international collaboration is working on standardization of detection as well as quantitation of microparticles by flow cytometry. Although it has evolved as an important vascular biology research tool, microparticle detection needs further evaluation and refinement before it becomes truly useful as a clinical tool.