PTBP1 knockdown in renal cell carcinoma inhibits cell migration, invasion and angiogenesis in vitro and metastasis in vivo via the hypoxia inducible factor-1 pathway
PTBP1 knockdown in renal cell carcinoma inhibits cell migration, invasion and angiogenesis in vitro and metastasis in vivo via the hypoxia inducible factor-1 pathway
复制标题
肾细胞癌中 PTBP1 敲低通过缺氧诱导因子 1 途径抑制体外细胞迁移、侵袭和血管生成以及体内转移
DOI：
10.3892/ijo.2018.4296
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发表时间：
2018-05-01
影响因子：
5.2
通讯作者：
Zheng, Junnian
中科院分区：
文献类型：
--
作者：
Shan, Haixia;Hou, Pingfu;Zheng, Junnian
聚嘧啶束结合蛋白1 （polypy嘧啶束结合蛋白1，PTBP1）是一种异构核核糖核蛋白，是一种多功能rna结合蛋白。PTBP1参与许多生物过程，包括维持细胞结构和运动、免疫、蛋白质代谢和细胞周期。本研究旨在探讨PTBP1表达与肾细胞癌（RCC）患者预后及临床病理特征的关系。并探讨了PTBP1在RCC转移中的潜在作用机制。结果表明，与正常肾组织相比，PTBP1在RCC组织中过表达。PTBP1表达与患者预后呈负相关，与肿瘤大小、病理性肿瘤（pT）、病理性转移（pM）状态、肿瘤淋巴结转移（TNM）分期呈正相关。体外敲低PTBP1可抑制RCC细胞的迁移、侵袭、增殖和血管生成，表明PTBP1主要通过缺氧诱导因子-1途径影响RCC细胞。此外，pptbp1基因敲低可减少体内RCC肺转移。本研究表明，PTBP1基因敲低可抑制肿瘤的进展和转移，提示PTBP1是影响RCC预后的重要因素，有望成为治疗RCC的新方法。
Polypyrimidine tract-binding protein 1 (PTBP1), a heterogeneous nuclear ribonucleoprotein, is a multi-functional RNA-binding protein. PTBP1 participates in a number of biological processes, including maintaining cell structure and motility, immunity, protein metabolism and the cell cycle. The present study aimed to investigate the association between PTBP1 expression and the prognosis and clinicopathological characteristics of patients with renal cell cancer (RCC). The potential mechanism of action of PTBP1 in the metastasis of RCC was also investigated. The results demonstrated that PTBP1 was overexpressed in RCC tissues compared with normal renal tissues. Furthermore, PTBP1 expression was negatively associated with patient prognosis and positively associated with tumor size, pathological tumor (pT) and pathological metastasis (pM) status and tumor lymph node metastasis (TNM) stage. PTBP1 knockdown in vitro inhibited RCC cell migration, invasion, proliferation and angiogenesis, and it was demonstrated that PTBP1 affected RCC cells primarily via the hypoxia inducible factor-1 pathway. Furthermore, PTBP1 knockdown decreased RCC lung metastasis in vivo. The present study demonstrated that PTBP1 knockdown suppresses tumor progression and metastasis, indicating that PTBP1 is an important prognostic factor in RCC and that it may be developed as a novel method of treating patients with RCC.