New 3′ elements control Pax6 expression in the developing pretecturn, neural retina and olfactory region
New 3′ elements control Pax6 expression in the developing pretecturn, neural retina and olfactory region
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DOI：
10.1016/s0925-4773(01)00646-3
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发表时间：
2002-03-01
影响因子：
2.6
通讯作者：
van Heyningen, V
中科院分区：
文献类型：
--
作者：
Griffin, C;Kleinjan, DA;van Heyningen, V
Pax 6是眼、嗅觉系统、前脑、垂体小脑、脊髓和胰腺发育的关键转录调控因子。选择性剪接、启动子使用和多个增强子调控复杂的Pax 6时空表达模式。染色体重排废除PAX 6基因表达的特点是下游的编码区。通过进化序列比较和转基因报告基因研究，我们已经确定了一个新的Pax 6 3'顺式调控区。该区域，C1170框123，包含三个不同的模块的人-小鼠序列保守，而只有框1是保守的河豚。无论是人类和orthopathic河豚序列直接类似的报告基因表达的发展pretectum，神经视网膜和嗅觉区，表明进化保守的Pax 6调控机制，尽管整体序列保守水平低。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
Pax6 is a key transcriptional regulator in eye, olfactory system, forebrain, pituitary cerebellum, spinal cord and pancreas development. Alternative splicing, promoter usage and multiple enhancers regulate the complex Pax6 spatio-temporal expression pattern. Chromosomal rearrangements which abolish PAX6 gene expression have been characterised downstream of the coding region. Through evolutionary sequence comparison and transgenic reporter studies, we have identified a new Pax6 3' cis-regulatory region. This region, C1170 Box 123, contains three distinct modules of human-mouse sequence conservation, while only Box 1 is conserved to Fugu. Both the human and the orthologous Fugu sequence direct similar reporter gene expression in the developing pretectum, neural retina and olfactory region, indicating evolutionary conservation of Pax6 regulatory mechanisms despite the low level of overall sequence conservation. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.