A family of chimeric erythrocyte binding proteins of malaria parasites
A family of chimeric erythrocyte binding proteins of malaria parasites
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DOI：
10.1073/pnas.95.3.1230
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发表时间：
1998-02-03
影响因子：
11.1
通讯作者：
Adams, JH
中科院分区：
文献类型：
--
作者：
Kappe, SHI;Noe, AR;Adams, JH
疟疾裂殖子顶端复合体细胞器中隔离的蛋白质参与了红细胞的入侵，但很少有这些蛋白质及其与宿主红细胞的相互作用被表征。在这份报告中，我们描述了MAEBL，一个在啮齿动物疟疾寄生虫约氏疟原虫和伯氏疟原虫中发现的红细胞结合蛋白家族。MAEBL具有嵌合性，将来自两个不同顶端细胞器蛋白家族的结构域连接到一个蛋白质家族中，MAEBL具有与位于裂殖子微核中的红细胞结合蛋白Duffy结合蛋白家族同源的分子结构，然而，MAEBL的氨基半胱氨酸富含结构域与普遍认同的Duffy结合样氨基半胱氨酸配基结构域不相似，而类似于顶膜抗原1(AMA-1)棒状蛋白家族的44 kDa外区片段，EBMAL在体外表达时具有该AMA-L样结构域的串联重复，并且这两个富含半胱氨酸的结构域在体外表达时都与红细胞结合Yoelii yoelii的ym克隆发生了maeb1基因的差异转录和剪接。MAEBL的顶端分布表明MAEBL定位于顶端复合体的锥体细胞器中，我们认为MAEBL是参与宿主细胞入侵的疟原虫红细胞结合蛋白家族中高度保守的成员。
Proteins sequestered within organelles of the apical complex of malaria merozoites are involved in erythrocyte invasion, but few of these proteins and their interaction with the host erythrocyte have been characterized. In this report we describe MAEBL, a family of erythrocyte binding proteins identified in the rodent malaria parasites Plasmodium yoelii yoelii and Plasmodium berghei. MAEBL has a chimeric character, uniting domains from two distinct apical organelle protein families within one protein, MAEBL has a molecular structure homologous to the Duffy binding-like family of erythrocyte binding proteins located in the micronemes of merozoites, However, the amino cysteine-rich domain of MAEBL has no similarity to the consensus Duffy binding-like amino cysteine-rich ligand domain, but instead is similar to the 44-kDa ectodomain fragment of the apical membrane antigen 1 (AMA-1) rhoptry protein family, MAEBL has a tandem duplication of this AMA-l-like domain, and both of these cysteine-rich domains bound erythrocytes when expressed in vitro, Differential transcription and splicing of the maebl locus occurred in the YM clone of P, yoelii yoelii. The apical distribution of MAEBL suggested localization within the rhoptry organelles of the apical complex, We propose that MAEBL is a member of a highly conserved family of erythrocyte binding proteins of Plasmodium involved in host cell invasion.