Determining the position of the cell division plane
Determining the position of the cell division plane
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DOI：
10.1038/nature01860
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发表时间：
2003-08-28
期刊：
影响因子：
64.8
通讯作者：
Salmon, ED
中科院分区：
文献类型：
--
作者：
Canman, JC;Cameron, LA;Salmon, ED
有丝分裂过程中细胞分裂面的正确定位对于确定两个子细胞的大小和位置至关重要，这是发育和细胞分化过程中的关键步骤(1)。已提出双极微管阵列是哺乳动物细胞中沟槽定位的最低要求，在纺锤体极之间微管正端重叠的部位形成沟槽(2-4)。然而，对其他物种的观察表明，这可能不是真的(5,6)。在这里，我们表明，通过诱导具有单极纺锤体的哺乳动物组织细胞进入后期(7,8)，培养的哺乳动物细胞中的沟槽形成不需要双极纺锤体。出乎意料的是，胞质分裂在单极细胞中频繁发生。分裂总是发生在染色体远端的皮质位置。对单极和双极纺锤体细胞胞质分裂过程中微管的分析表明，稳定的微管亚群延伸穿过染色体并在皱纹形成部位与细胞皮层结合。我们的数据与染色体为微管提供促进微管稳定性和皱纹的因子的模型一致。
Proper positioning of the cell division plane during mitosis is essential for determining the size and position of the two daughter cells-a critical step during development and cell differentiation(1). A bipolar microtubule array has been proposed to be a minimum requirement for furrow positioning in mammalian cells, with furrows forming at the site of microtubule plus-end overlap between the spindle poles(2-4). Observations in other species have suggested, however, that this may not be true(5,6). Here we show, by inducing mammalian tissue cells with monopolar spindles to enter anaphase(7,8), that furrow formation in cultured mammalian cells does not require a bipolar spindle. Unexpectedly, cytokinesis occurs at high frequency in monopolar cells. Division always occurs at a cortical position distal to the chromosomes. Analysis of microtubules during cytokinesis in cells with monopolar and bipolar spindles shows that a subpopulation of stable microtubules extends past chromosomes and binds to the cell cortex at the site of furrow formation. Our data are consistent with a model in which chromosomes supply microtubules with factors that promote microtubule stability and furrowing.