High Expression of Kruppel-like Factor 7 Indicates Unfavorable Clinical Outcomes in Patients with Lung Adenocarcinoma
High Expression of Kruppel-like Factor 7 Indicates Unfavorable Clinical Outcomes in Patients with Lung Adenocarcinoma
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DOI：
10.1016/j.jss.2019.12.053
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发表时间：
2020-06-01
影响因子：
2.2
通讯作者：
Jiang, Daowen
中科院分区：
文献类型：
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作者：
Niu, Rungui;Tang, Yanlei;Jiang, Daowen
背景：Kru pel-like factor 7 （KLF7）属于锌指转录因子KLF家族，在调控基因表达中起关键作用。据报道，KLF7过表达与胃癌的进展密切相关。然而，KLF7在肺腺癌（LAC）中的作用尚未阐明。我们的研究目的是研究KLF7的表达模式，并探讨KLF7的表达是否与LAC患者的不良临床结局相关。材料和方法：采用免疫组化染色法和定量逆转录聚合酶链反应法检测LAC组织中KLF7蛋白和mRNA水平。通过单因素和多因素分析评估KLF7在LAC患者中的预后作用。采用Kaplan-Meier分析和logrank检验评价临床疗效。通过细胞实验研究KLF7对肺癌细胞的作用。结果：与周围正常组织相比，LAC组织中KLF7表达明显升高。KLF7蛋白水平高与肿瘤大小较大、淋巴结转移阳性、TNM分期晚期相关。此外，KLF7表达水平较高的LAC患者总生存期较差，KLF7被认为是一个不利的独立预后因素。敲低KLF7可以抑制癌细胞的增殖和侵袭能力。结论：我们的研究表明，高水平的KLF7表达与LAC患者较差的临床预后显著相关，表明KLF7作为一种新的预后生物标志物和治疗靶点的潜在作用。(C) 2020爱思唯尔公司版权所有。
Background: Kru ppel-like factor 7 (KLF7), which belongs to the KLF family of zinc finger transcription factors, plays a critical role in regulating gene expression. It was reported that KLF7 overexpression was closely related to the progression of gastric cancer. However, the role of KLF7 in lung adenocarcinoma (LAC) has not been elucidated. The aim of our study is to investigate the expression pattern of KLF7 and explore whether the KLF7 expression is correlated with unfavorable clinical outcome of patients with LAC. Materials and methods: The protein and mRNA levels of KLF7 were examined in LAC tissues by using immunohistochemistry staining and quantitative reverse transcription polymerase chain reaction, respectively. The prognostic role of KLF7 in patients with LAC was assessed using univariate and multivariate analyses. Clinical outcomes were evaluated by Kaplan-Meier analysis and logrank test. The effects of KLF7 on lung cancer cells were investigated through cellular experiments. Results: KLF7 expression was elevated in LAC tissues compared with adjacent normal tissues. High protein level of KLF7 was correlated with larger tumor size, positive lymph node metastasis, and advanced TNM stage. Moreover, patients with LAC with higher expression level of KLF7 had poorer overall survival, and KLF7 was identified as an unfavorable independent prognosis factor. Knockdown of KLF7 can suppress the proliferation and invasion abilities of cancer cells. Conclusions: Our studies revealed that high KLF7 expression level was significantly associated with the poorer clinical outcomes of patients with LAC, indicating the potential role of KLF7 as a novel prognostic biomarker and therapeutic target. (C) 2020 Elsevier Inc. All rights reserved.