Rostral ventrolateral medullary opioid receptor subtypes in the inhibitory effect of electroacupuncture on reflex autonomic response in cats
Rostral ventrolateral medullary opioid receptor subtypes in the inhibitory effect of electroacupuncture on reflex autonomic response in cats
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DOI：
10.1016/s1566-0702(01)00247-8
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发表时间：
2001-06-20
影响因子：
2.7
通讯作者：
Longhurst, JC
中科院分区：
文献类型：
--
作者：
Li, P;Tjen-A-Looi, S;Longhurst, JC
电针（EA）在中医中用于治疗心律失常、高血压和心肌缺血。我们之前的研究表明，EA对缓激肽（BK）作用于胆囊引起的压力反射的抑制作用，部分是由于阿片受体的激活，最有可能位于延髓吻侧腹侧（rVLM）。然而，具体的阿片受体亚型，因此神经递质，负责这种抑制是未知的。因此，在麻醉的猫中，将BK（10杯/毫升）应用于胆囊以诱导动脉血压（BP）的短暂反射性升高。电针（1-2 mA, 5 Hz， 0.5 ms脉冲）通过双侧针刺于前肢正中神经上方的内关穴和健斯基穴。EA使bk诱导的压力反应减弱39%。阿片受体亚型拮抗剂或激动剂单侧微注射入rVLM。受体拮抗剂CTOP和受体拮抗剂ICI分别能显著减弱ea诱导的抑制作用至少30分钟。kappa受体拮抗剂（无bni）效果较差，作用时间较短。与EA一样，在rVLM中分别微量注射-阿片受体激动剂DAGO和-阿片受体激动剂DADLE可显著降低压力反应。相比之下，kappa -阿片激动剂U50,488未能改变bk诱导的压力反应。我们得出结论，EA抑制胆囊压力反应的很大一部分与rVLM中mu-和delta -阿片受体的激活有关。内源性阿片受体和阿片受体的神经递质，内啡肽和脑啡肽，在rVLM中，似乎在ea相关的心血管rifler反应调节中发挥作用。相反，dynorphin不太可能参与这种反应。(C) 2001 Elsevier Science B.V.版权所有
Electroacupuncture (EA) is used in traditional Chinese medicine to treat arrhythmias, hypertension and myocardial ischemia. Our previous work suggests that the inhibitory effect of EA on the presser reflex induced by bradykinin (BK) applied to the gallbladder is due, in part, to the activation of opioid receptors, most likely located in the rostral ventrolateral medulla (rVLM). However, specific opioid receptor subtypes, and hence the neurotransmitters, responsible for this inhibition are unknown. Therefore, in anesthetized cats, BK (10 mug/ml) was applied to the gallbladder to induce transient reflex increases in arterial blood pressure (BP). EA (1-2 mA, 5 Hz, 0.5 ms pulses) was delivered through acupuncture needles inserted bilaterally into Neiguan and Jianski acupoints on forelimbs, overlying the median nerves. EA attenuated the BK-induced presser response by 39%. Opioid receptor subtype antagonists or agonists were microinjected unilaterally into the rVLM. The mu- and delta -receptor antagonists CTOP and ICI 174,864, respectively, significantly attenuated the EA-induced inhibition for at least 30 min. The kappa -receptor antagonist (nor-BNI) was less effective and was shorter acting. Like EA, microinjection of mu- and delta -opioid agonists, DAGO and DADLE, respectively, into the rVLM significantly decreased the presser responses. In contrast, the kappa -opioid agonist, U50,488, failed to alter the BK-induced presser response. We conclude that a significant portion of inhibition of the gallbladder presser response: by EA is related to activation of mu- and delta -opioid receptors in the rVLM. The endogenous neurotransmitters for mu- and delta -opioid receptors, beta -endorphins and enkephalins, in the rVLM, therefore appear to play a role in the EA-related modulation of cardiovascular rifler responses. Conversely, dynorphin is less likely to be involved in this response. (C) 2001 Elsevier Science B.V. All rights reserved.