Blood Ketone Bodies and Breath Acetone Analysis and Their Correlations in Type 2 Diabetes Mellitus
Blood Ketone Bodies and Breath Acetone Analysis and Their Correlations in Type 2 Diabetes Mellitus
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DOI：
10.3390/diagnostics9040224
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发表时间：
2019-12-01
期刊：
影响因子：
3.6
通讯作者：
Mwakikunga, Bonex
中科院分区：
文献类型：
--
作者：
Saasa, Valentine;Beukes, Mervyn;Mwakikunga, Bonex
呼气中挥发性有机化合物的分析由于其快速的检测结果和非侵入性，特别是对糖尿病(DM)的检测，已经获得了发展势头和临床意义。研究表明，呼气气体，包括丙酮，可能与成人2型和1型糖尿病患者的血糖(BG)和血酮水平有关。检测呼吸、血液和尿液中酮的浓度变化可能对糖尿病的诊断和监测至关重要。这项研究评估了简单的呼吸测试作为一种非侵入性的糖尿病监测手段在成人2型糖尿病患者中的有效性。人体呼气样品采集在Tedlar(TM)袋中，采用顶空固相微萃取-气相色谱-质谱仪(HS-SPME/GC-MS)。对30名2型糖尿病患者和28名对照志愿者进行常规医院门诊时同时测定的毛细血管血糖和血酮(β-羟丁酸和乙酰乙酸乙酯)水平的比较。与对照组相比，糖尿病受试者的酮体显著增加；然而，糖尿病和非糖尿病志愿者的酮体水平都得到了控制。值得注意的是，呼气丙酮与血乙酰乙酸酯(R=0.89)以及呼气丙酮与β-羟基丁酸酯(R=0.82)之间存在统计学意义上的相关性。
Analysis of volatile organic compounds in the breath for disease detection and monitoring has gained momentum and clinical significance due to its rapid test results and non-invasiveness, especially for diabetes mellitus (DM). Studies have suggested that breath gases, including acetone, may be related to simultaneous blood glucose (BG) and blood ketone levels in adults with types 2 and 1 diabetes. Detecting altered concentrations of ketones in the breath, blood and urine may be crucial for the diagnosis and monitoring of diabetes mellitus. This study assesses the efficacy of a simple breath test as a non-invasive means of diabetes monitoring in adults with type 2 diabetes mellitus. Human breath samples were collected in Tedlar (TM) bags and analyzed by headspace solid-phase microextraction and gas chromatography-mass spectrometry (HS-SPME/GC-MS). The measurements were compared with capillary BG and blood ketone levels (beta-hydroxybutyrate and acetoacetate) taken at the same time on a single visit to a routine hospital clinic in 30 subjects with type 2 diabetes and 28 control volunteers. Ketone bodies of diabetic subjects showed a significant increase when compared to the control subjects; however, the ketone levels were was controlled in both diabetic and non-diabetic volunteers. Worthy of note, a statistically significant relationship was found between breath acetone and blood acetoacetate (R = 0.89) and between breath acetone and beta-hydroxybutyrate (R = 0.82).