Lipid diversity among botulinum neurotoxin-producing clostridia
Lipid diversity among botulinum neurotoxin-producing clostridia
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DOI：
10.1099/mic.0.060707-0
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发表时间：
2012-10-01
期刊：
影响因子：
2.8
通讯作者：
Goldfine, Howard
中科院分区：
文献类型：
--
作者：
Guan, Ziqiang;Johnston, Norah C.;Goldfine, Howard
肉毒梭菌根据其生理特性和16 S rRNA测序分为四组（I至IV组）。本文对11株代表性的C.所有菌株均含有磷脂酰甘油（PG）、心磷脂（CL）和磷脂酰乙醇胺（PE），其均为全酰基和链烯基（缩醛磷脂）形式。蛋白水解群I中5株菌株与C.产孢梭菌中含有不同量的N-乙酰葡糖胺基-二酰基甘油的乙醇胺-磷酸衍生物，其也存在于C.产孢菌组II中的三种菌株与丁酸梭菌、拜氏梭菌和丙酮丁醇梭菌有关，含有这些糖解物种的脂质特征：PE的缩醛磷脂形式的甘油缩醛和PG缩醛。与诺维梭菌相关的两个III组菌株含有PG的氨基酰基衍生物，其也在C.诺维。组IV中的菌株具有PE、PG和CL，但没有可区分的脂质。本工作表明，C.肉毒杆菌与其他方法的分类一致。
Clostridium botulinum has been classified into four groupings (groups I to IV) based on physiological characteristics and 16S rRNA sequencing. We have examined the lipid compositions of 11 representative strains of C. botulinum and a strain of Clostridium sporogenes by 2D-TLC and by MS. All strains contained phosphatidylglycerol (PG), cardiolipin (CL) and phosphatidylethanolamine (PE) in both the all-acyl and the alk-1'-enyl (plasmalogen) forms. Five strains in proteolytic group I, which are related to C. sporogenes, contained varying amounts of an ethanolamine-phosphate derivative of N-acetylglucosaminyl-diradylglycerol, which is also present in C. sporogenes. Three strains in group II, which are related to Clostridium butyricum, Clostridium beijerinckii and Clostridium acetobutylicum, contained lipids characteristic of these saccharolytic species: a glycerol acetal and a PG acetal of the plasmalogen form of PE. Two group III strains, which are related to Clostridium novyi, contained amino-acyl derivatives of PG, which are also found in C. novyi. A strain in group IV had PE, PG and CL, but none of the distinguishing lipids. This work shows that the lipidome of C. botulinum is consistent with its classification by other methods.