Biochemical and biophysical studies of Bacillus subtilis envelopes under hyperosmotic stress.
Biochemical and biophysical studies of Bacillus subtilis envelopes under hyperosmotic stress.
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DOI：
10.1016/s0168-1605(00)00171-9
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发表时间：
2000-04
影响因子：
5.4
通讯作者：
C. López;H. Heras;H. Garda;S. Ruzal;C. Sánchez-Rivas;E. Rivas
中科院分区：
文献类型：
--
作者：
C. López;H. Heras;H. Garda;S. Ruzal;C. Sánchez-Rivas;E. Rivas
比较了在含有和不含 1.5 M NaCl 的 Luria Bertani (LB) 培养基中生长的枯草芽孢杆菌培养物的包膜的行为和状态。在高渗条件下，培养物的疏水性增加。磷脂和脂肪酸 (FA) 组成显示出重要的差异：更高的心磷脂 (CL) 含量 [以磷脂酰甘油 (PG) 为代价]，以及更高的不饱和和直链 FA 含量。用荧光探针测定的膜的流动性表明细胞质膜的粘度增加。讨论了膜渗透性和渗透压耐受性的这些变化的后果。
The behaviour and state of the envelopes from B. subtilis cultures grown in Luria Bertani (LB) medium with and without 1.5 M NaCl are compared. Under hypertonic conditions, the hydrophobicity of the cultures increases. The phospholipid and fatty acid (FA) compositions show important differences: a higher cardiolipin (CL) content [at the expense of phosphatidylglycerol (PG)], and a higher unsaturated and straight chain FA content. The fluidity of the membranes, determined with fluorescent probes, indicates an increase in viscosity of the cytoplasmic membrane. The consequences of these variations in membrane permeability and osmotolerance are discussed.