Deep-sea vent ε-proteobacterial genomes provide insights into emergence of pathogens
Deep-sea vent ε-proteobacterial genomes provide insights into emergence of pathogens
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DOI：
10.1073/pnas.0700687104
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发表时间：
2007-07-17
影响因子：
11.1
通讯作者：
Horikoshi, Koki
中科院分区：
文献类型：
--
作者：
Nakagawa, Satoshi;Takaki, Yoshihiro;Horikoshi, Koki
深海喷口是一种不依赖光、高产的生态系统，主要由趋化岩石自养微生物驱动，特别是与重要病原体系统发育相关的e -变形杆菌。我们分析了两种深海火山口epsilon-Proteo细菌菌株Sulfurovum， sp. NBC37-1和Nitratiruptor sp. SB155-2的基因组，这不仅提供了它们在海底不同寻常的生态位的见解，而且还提供了它们的致病亲缘物种Helicobacter和Campylobacter的毒力起源。深海喷口e-变形菌基因组编码呼吸、感知和响应环境、重金属解毒等多个系统，反映了它们对深海喷口环境的适应性。虽然它们是非致病性的，但这两种深海喷口epsilon-Proteo细菌都与致病性E-Proteobacteria具有许多毒力基因，包括毒力因子MviN、溶血素、入侵抗原CiaB和n-连锁糖基化基因簇的基因。此外，致病后代的一些毒力决定因素（如h -2摄取氢化酶）和基因组可塑性似乎源于深海喷口e-变形菌。这为深海热泉喷口的epsilon-变形杆菌提供了生态优势，它们在深海栖息地茁壮成长，对其致病亲缘菌的有效定植和持续感染至关重要。我们的比较基因组分析表明，在重要的人类/动物病原体与其非致病性、共生的、趋化岩石自养的深海亲戚之间存在着以前未被认识到的进化联系。
Deep-sea vents are the light-independent, highly productive ecosystems driven primarily by chemolithoautotrophic microorganisms, in particular by E-Proteobacteria phylogenetically related to important pathogens. We analyzed genomes of two deep-sea vent epsilon-Proteo bacteria strains, Sulfurovum, sp. NBC37-1 and Nitratiruptor sp. SB155-2, which provide insights not only into their unusual niche on the seafloor, but also into the origins of virulence in their pathogenic relatives, Helicobacter and Campylobacter species. The deep-sea vent e-proteobacterial genomes encode for multiple systems for respiration, sensing and responding to environment, and detoxifying heavy metals, reflecting their adaptation to the deep-sea vent environment. Although they are nonpathogenic, both deep-sea vent epsilon-Proteo bacteria share many virulence genes with pathogenic E-Proteobacteria, including genes for virulence factor MviN, hemolysin, invasion antigen CiaB, and the N-linked glycosylation gene cluster. In addition, some virulence determinants (such as the H-2-uptake hydrogenase) and genomic plasticity of the pathogenic descendants appear to have roots in deep-sea vent e-Proteobacteria. These provide ecological advantages for hydrothermal vent epsilon-Proteobacteria who thrive in their deep-sea habitat and are essential for both the efficient colonization and persistent infections of their pathogenic relatives. Our comparative genomic analysis suggests that there are previously unrecognized evolutionary links between important human/animal pathogens and their nonpathogenic, symbiotic, chemolithoautotrophic deepsea relatives.