INFLUENCE OF TEMPERATURE ON VIRULENCE OF RACE 3 STRAINS OF PSEUDOMONAS-SOLANACEARUM
INFLUENCE OF TEMPERATURE ON VIRULENCE OF RACE 3 STRAINS OF PSEUDOMONAS-SOLANACEARUM
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DOI：
10.1007/bf02854025
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
SEQUEIRA, L
中科院分区：
文献类型：
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作者：
CIAMPI, L;SEQUEIRA, L
在平均气温相对较低的哥伦比亚和秘鲁高海拔地区，由青枯菌 3 号小种引起的马铃薯青枯病可能是一个严重问题。由于小种 3 存在于广泛的地理区域，因此尝试确定是否存在适应这些低温并能在这些低温下引起枯萎的特定菌株。用8个小种3菌株对“Russet Burbank”马铃薯植物进行茎接种，然后将其维持在16、20、24和28℃的生长室中。 C 15天。在最低温度（16°C）下，只有 1 个菌株（S206）引起相当程度的枯萎； S206在16和20℃下均比其他7株毒力更强。 C. 28 度。 C、未检测到毒力差异；所有菌株均在 15 天后完全枯萎。这些结果在第二个实验中得到了证实，其中使用了 10 个菌株，其中 5 个为小种 1，5 个为小种 3。只有 2 个小种 3 菌株（S206 和 257）在较低温度下引起枯萎。 28 度。 C 所有菌株均具有同等毒性。毒力差异与16℃液体培养中菌株的生长速率不相关。 C;一些菌株在体外的生长速度与 S206 或 257 相似，但在该温度下体内生长时不会引起症状。
The bacterial wilt of potatoes caused by race 3 of P. solanacearum can be a severe problem at high elevations in Colombia and Peru, where relatively low mean temperatures predominate. Because race 3 exists within wide geographical areas, attempts were made to determine if there are specific strains that are adapted to, and can cause wilting at, these low temperatures. ''Russet Burbank'' potato plants were stem-inoculated with 8 race 3 strains and then were maintained in growth chambers at 16, 20, 24 and 28.degree. C for 15 days. At the lowest temperature (16.degree. C) only 1 strain (S206) caused considerable wilting; S206 was more virulent than the other 7 strains at both 16 and 20.degree. C. At 28.degree. C, no differences in virulence could be detected; all strains caused complete wilting by 15 days. These results were confirmed in a 2nd experiment in which 10 strains were used, 5 of race 1 and 5 of race 3. Only 2 race 3 strains (S206 and 257) caused wilting at the lower temperatures; at 28.degree. C all strains were equally virulent. Differences in virulence were not correlated with growth rates of the strains in liquid culture at 16.degree. C; some strains grew at rates similar to those of S206 or 257 in vitro, but did not cause symptoms when grown in vivo at this temperature.