Effect of dietary Bacillus subtilis on proportion of Bacteroidetes and Firmicutes in swine intestine and lipid metabolism
Effect of dietary Bacillus subtilis on proportion of Bacteroidetes and Firmicutes in swine intestine and lipid metabolism
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DOI：
10.4238/2013.may.23.1
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发表时间：
2013-01-01
影响因子：
0.4
通讯作者：
Wang, K. N.
中科院分区：
文献类型：
--
作者：
Cui, C.;Shen, C. J.;Wang, K. N.
肠道中类杆菌和细菌群的比例会影响吸收营养的能力。研究了日粮中添加益生菌枯草芽孢杆菌对肥育猪生长性能、脂肪沉积、血脂、拷贝数、盲肠内容物中类杆菌和类杆菌的比例以及肝脏和脂肪组织中关键脂代谢酶基因表达的影响。选用8周龄杜×眉山杂交猪24头(10.28+/-0.59 kg)，随机分为两组：添加枯草杆菌的玉米-豆粕日粮(益生菌组)和不添加枯草杆菌的玉米-豆粕日粮(对照组)。益生菌日粮能显著提高10~110 kg猪的平均日增重和饲料转化率。添加益生菌后，猪的平均背脂深度增加，而猪油叶重降低。摄入益生菌可降低血清甘油三酯和葡萄糖浓度，但不改变血清总胆固醇和游离脂肪酸水平。日粮中添加益生菌可下调肝脏脂肪酸合成酶(Fas)和乙酰辅酶A羧基酶α(ACCα)的mRNA表达。反之，脂肪组织中Fas和ACCα的基因表达增加。益生菌饲料降低了拟杆菌的拷贝数和百分比，而增加了盲肠内容物中非米米特的百分比。我们的结论是，添加枯草杆菌可以改善肥育猪的生长性能，上调皮下脂肪中的脂肪代谢。我们得出结论，枯草杆菌通过调节肠道中类杆菌和细菌的比例来影响脂肪代谢。
The ratio of Bacteroidetes and Firmicutes bacterial groups in the gut can affect the ability to absorb nutrients. We investigated the effect of probiotic Bacillus subtilis supplementation of diets on growth performance, fat deposition, blood lipids, copy numbers, and percentage of Bacteroidetes and Firmicutes in cecal contents, as well as mRNA expression of key lipid metabolism enzymes in the liver and adipose tissue of finishing pigs. Twenty-four Duroc x Meishan crossbreed 8-week-old pigs (10.28 +/- 0.59 kg) were randomly allocated to two dietary treatments: maize-soybean meal-based diets with B. subtilis (probiotic group) and without B. subtilis (control group). The probiotic diet led to a significant increase in the average daily gain and feed conversion ratio of pigs weighing 10 to 110 kg. The mean backfat depth was increased while leaf lard weights were decreased by probiotic supplementation. Ingestion of probiotics decreased the serum triglyceride and glucose concentrations, but did not change the levels of total cholesterol and free fatty acids in the serum. The mRNA expressions of fatty acid synthase (FAS) and acetyl-CoA carboxylase alpha (ACC alpha) in the liver were down-regulated by the dietary probiotic supplement. Conversely, the gene expressions of FAS and ACC alpha in the adipose tissue increased. The probiotic diet decreased the copy numbers and percentage of Bacteroidetes, while it increased the percentage of Firmicutes in the cecal contents. We conclude that the addition of B. subtilis improves growth performance and up-regulates lipid metabolism in subcutaneous fat of finishing pigs. We conclude that B. subtilis affects lipid metabolism through regulation of the proportion of Bacteroidetes and Firmicutes in the gut.