Molecular, serological and in vitro culture-based characterization of Bourbon virus, a newly described human pathogen of the genus Thogotovirus
Molecular, serological and in vitro culture-based characterization of Bourbon virus, a newly described human pathogen of the genus Thogotovirus
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DOI：
10.1016/j.jcv.2015.10.021
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发表时间：
2015-12-01
影响因子：
8.8
通讯作者：
Kosoya, Olga I.
中科院分区：
文献类型：
--
作者：
Lambert, Amy J.;Velez, Jason O.;Kosoya, Olga I.
背景资料：2014年6月，一名来自堪萨斯的健康男子因近期接触蜱虫而死于与发热、血小板减少和白细胞减少症有关的并发症。在诊断检测过程中，从该患者的血液中分离出一株分离株。该分离株随后被确定为一种新的正粘病毒属Thogotovirus的下一代测序，并命名为波旁病毒后，病人的县residence.Objectives：为了支持研究和诊断的目的，我们提供了一个基本的描述波旁病毒的分子和血清学水平。此外，为了初步确定潜在的宿主和载体范围关联，我们表征了波旁病毒在多种脊椎动物和无脊椎动物细胞系中的生长动力学。研究设计：对波旁病毒进行下一代高通量测序、系统发育和基本结构蛋白分析以及双向空斑减少中和试验。此外，我们接种了各种类型的细胞与波旁病毒，并通过确定病毒滴度的生长动力学进行评估，从感染的细胞随时间推移的上清液中。结果：波旁病毒具有24-82%的氨基酸序列水平的同一性和低血清学交叉反应性与其他Thogotoviruses。在体外生长动力学显示强大的复制波旁病毒在哺乳动物和蜱celles.Conclusions：分子和血清学特征识别波旁病毒属Thogotovirus的一个新成员。细胞培养分析结果表明，波旁病毒与哺乳动物和蜱宿主之间存在关联。由爱思唯尔公司出版
Background: In June of 2014, a previously healthy man from Kansas with a recent history of tick exposure died from complications related to an illness marked by fever, thrombocytopenia and leukopenia. An isolate was derived from the blood of this patient during the course of diagnostic testing. This isolate was subsequently identified as a novel orthomyxovirus of the genus Thogotovirus by next generation sequencing and was named Bourbon virus after the patient's county of residence.Objectives: To support research and diagnostic aims, we provide a basic description of Bourbon virus at both the molecular and serological levels. Furthermore, to preliminarily identify potential host and vector range associations we have characterized the growth kinetics of Bourbon virus in a variety of vertebrate and invertebrate cell lines.Study design: Bourbon virus was subjected to next generation-high throughput sequencing, phylogenetic, and basic structural protein analyses as well as 2-way plaque reduction neutralization assays. Also, we inoculated a variety of cell types with Bourbon virus and evaluated the growth kinetics by determining viral titers in the supernatants taken from infected cells over time.Results: Bourbon virus possesses 24-82% identity at the amino acid sequence level and low serological cross-reactivity with other Thogotoviruses. In vitro growth kinetics reveal robust replication of Bourbon virus in mammalian and tick cells.Conclusions: Molecular and serological characterizations identify Bourbon virus as a novel member of the genus Thogotovirus. Results from cell culture analyses suggest an association between Bourbon virus and mammalian and tick hosts. Published by Elsevier B.V.