Borrelia miyamotoi infection leads to cross-reactive antibodies to the C6 peptide in mice and men
Borrelia miyamotoi infection leads to cross-reactive antibodies to the C6 peptide in mice and men
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DOI：
10.1016/j.cmi.2019.07.026
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发表时间：
2020-04-01
影响因子：
14.2
通讯作者：
Hovius, J. W.
中科院分区：
文献类型：
--
作者：
Koetsveld, J.;Platonov, A. E.;Hovius, J. W.
目的：宫本疏螺旋体是一种复发性热病疏螺旋体，由硬体硬蜱传播，也是伯氏疏螺旋体的主要传播媒介。一种广泛用于莱姆疏螺旋体病血清学诊断的试验是一种基于伯氏杆菌sl VlsE蛋白C6肽的酶免疫分析(EIA)。我们在宫本杆菌病(BMD)患者血清和实验性小鼠感染中研究了感染宫本杆菌后C6的反应性。方法：我们进行了电子分析，将C6多肽与免疫优势的宫本杆菌可变大蛋白(VLP)进行比较。接下来，我们检测了感染宫本杆菌的小鼠的血清中的C6反应性，以及来自46名BMD患者的191份独特的纵向集合的血清中的C6反应性。结果：在计算机分析中发现C6肽与宫本杆菌VLP中的结构域相似。在实验感染宫本杆菌的24只小鼠中，有21只证实了与C6肽的交叉反应。此外，46名BMD患者中有35名C6 EIA莱姆指数高于1.1(阳性)。有趣的是，37名C6EIA莱姆指数高于0.9(模棱两可)的患者中，有27人在使用商用免疫印迹检测伯氏杆菌sl抗体时呈阴性。结论：我们发现感染宫本杆菌会导致对C6多肽的交叉反应。由于BMD和莱姆型疏螺旋体病是在相同的地理位置发现的，当仅依靠C6反应性检测时应谨慎使用。我们建议，C6 EIA阳性，免疫印迹阴性，特别是在被扁虱叮咬几周后发烧的患者，需要进一步检测宫本芽孢杆菌。J.Koetsveld，Clin Microbiol Infect 2020；26：513.e1-513.e6(C)2019作者。由爱思唯尔有限公司代表欧洲临床微生物学和传染病学会出版。这是CC BY-NC-ND License(http://creativecommons.org/licenses/by-nc-nd/4.0/).下的一篇开放获取文章
Objectives: Borrelia miyamotoi is a relapsing fever Borrelia, transmitted by hard (Ixodes) ticks, which are also the main vector for Borrelia burgdorferi. A widely used test for serodiagnosis of Lyme borreliosis is an enzyme immunoassay (EIA) based on the C6 peptide of the B. burgdorferi sl VlsE protein. We set out to study C6 reactivity upon infection with B. miyamotoi in a large well-characterized set of B. miyamotoi disease (BMD) patient sera and in experimental murine infection.Methods: We performed in silico analyses, comparing the C6-peptide to immunodominant B. miyamotoi variable large proteins (Vlps). Next, we determined C6 reactivity in sera from mice infected with B. miyamotoi and in a unique longitudinal set of 191 sera from 46 BMD patients.Results: In silico analyses revealed similarity of the C6 peptide to domains within B. miyamotoi Vlps. Cross-reactivity against the C6 peptide was confirmed in 21 out of 24 mice experimentally infected with B. miyamotoi. Moreover, 35 out of 46 BMD patients had a C6 EIA Lyme index higher than 1.1 (positive). Interestingly, 27 out of 37 patients with a C6 EIA Lyme index higher than 0.9 (equivocal) were negative when tested for specific B. burgdorferi sl antibodies using a commercially available immunoblot.Conclusions: We show that infection with B. miyamotoi leads to cross-reactive antibodies to the C6 peptide. Since BMD and Lyme borreliosis are found in the same geographical locations, caution should be used when relying solely on C6 reactivity testing. We propose that a positive C6 EIA with negative immunoblot, especially in patients with fever several weeks after a tick bite, warrants further testing for B. miyamotoi. J. Koetsveld, Clin Microbiol Infect 2020;26:513.e1-513.e6 (c) 2019 The Authors. Published by Elsevier Ltd on behalf of European Society of Clinical Microbiology and Infectious Diseases. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).