Enhancement of glutamine utilization in Bacillus subtilis through the GlnK-GlnL two-component regulatory system
Enhancement of glutamine utilization in Bacillus subtilis through the GlnK-GlnL two-component regulatory system
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DOI：
10.1128/jb.187.14.4813-4821.2005
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发表时间：
2005-07-01
影响因子：
3.2
通讯作者：
Fujita, Y
中科院分区：
文献类型：
--
作者：
Satomura, T;Shimura, D;Fujita, Y
在DNA微阵列分析中，我们发现GlnK-GlnL（以前的YcbA-YcbB）双组分系统在北方分析中响应于培养基中的谷氨酰胺而正向调节glsA-glnT（以前的ybgJ-ybgH）操纵子的表达。作为凝胶阻滞和DNA酶I足迹分析的结果，我们发现GlnL蛋白与存在直接重复TTTTGTN 4 T TTTGT的区域（碱基-13至-56; +1是glsA-glnT的引物延伸分析确定的转录起始碱基）相互作用。此外，glsA和glnT基因被生物化学证实分别编码谷氨酰胺酶和谷氨酰胺转运蛋白。
During DNA microarray analysis, we discovered that the GlnK-GlnL (formerly YcbA-YcbB) two-component system positively regulates the expression of the glsA-glnT (formerly ybgJ-ybgH) operon in response to glutamine in the culture medium on Northern analysis. As a result of gel retardation and DNase I footprinting analyses, we found that the GlnL protein interacts with a region (bases -13 to -56; +1 is the transcription initiation base determined on primer extension analysis of glsA-glnT) in which a direct repeat, TTTTGTN4T TTTGT, is present. Furthermore, the glsA and glnT genes were biochemically verified to encode glutaminase and glutamine transporter, respectively.