Expression of vascular endothelial growth factor by photodynamic therapy with mono-L-aspartyl chlorin e6 (NPe6) in oral squamous cell carcinoma
Expression of vascular endothelial growth factor by photodynamic therapy with mono-L-aspartyl chlorin e6 (NPe6) in oral squamous cell carcinoma
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DOI：
10.1016/j.oraloncology.2006.03.020
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发表时间：
2007-07-01
期刊：
影响因子：
4.8
通讯作者：
Kimura, Hiroto
中科院分区：
文献类型：
--
作者：
Nakagawa, Hiroshi;Matsumiya, Tomoh;Kimura, Hiroto
光动力疗法（PDT）是用于治疗皮肤、膀胱和口腔的癌前病变和癌性病变的方法。然而，尽管初始反应良好，PDT后的肿瘤复发仍然是个问题。一些研究表明，PDT诱导血管内皮细胞。人口腔鳞状细胞生长因子（VEGF）的表达。癌和其他器官。然而，很少有人知道VEGF的表达应用于PDT在人癌细胞株。尚未在人口腔癌细胞系HSC-3细胞中进行使用第二代光敏剂Npe 6（Npe 6介导的PDT）的PDT的研究。我们研究了Npe 6介导的PDT对HSC-3细胞中VEGF、c-jun和c-fos原癌基因的表达的影响。我们还讨论了PDT诱导的氧化损伤可能通过VEGF表达导致血管生成反应的可能性。逆转录聚合酶链反应（RT-PCR）分析显示，Npe 6介导的PDT诱导VEGF，c-jun和c-fos的mRNA的表达在时间和浓度依赖性的方式。去铁胺（DFX），铁螯合剂，诱导VEGF的表达，但表达模式是不同的Npe 6介导的PDT。SB 203580、p38 MAPK抑制剂可抑制Npe 6介导的PDT诱导的VEGF、c-jun和c-fos mRNA的表达，蛋白质合成抑制剂放线菌酮（CHX）可抑制VEGF mRNA的表达。自由基清除剂N-乙酰-L-半胱氨酸（NAC）可抑制c-jun mRNA的表达，而增强c-fos mRNA的表达。结论：Npe 6介导的PDT诱导人口腔癌细胞系HSC-3表达VEGF、c-jun和c-fos，至少部分是通过激活p38 MAPK实现的。(c)2006年由Elsevier Ltd.出版
Photodynamic therapy (PDT) is a method for treating pre-cancerous and cancerous lesions of the skin, bladder and oral cavity. However, tumour recurrence after PDT remains problematic despite good initial response. Some studies have shown that PDT induces vascular endothelial. growth factor (VEGF) expression in human oral squamous cell. carcinoma and other organs. However, little is known about VEGF expression applied to PDT in human carcinoma cell tines. No studies have been conducted of PDT using Npe6 (Npe6-mediated PDT), a second-generation photosensitizer, in the human oral carcinoma cell line, HSC-3 cells. We investigated the expression of VEGF, c-jun and c-fos proto-oncogenes in HSC-3 cells in response to Npe6-mediated PDT. We also addressed the possibility that oxidative damage induced by PDT could lead to an angiogenic response, via VEGF expression. Reverse transcription polymerase chain reaction (RT-PCR) analysis, revealed that Npe6-mediated PDT induced the expression of mRNAs for VEGF, c-jun and c-fos in time- and concentration-dependent manners. Desferrioxamine (DFX), an iron chelator, induced VEGF expression, but the expression pattern was different to that of Npe6-mediated PDT. The expression mRNAs for VEGF, c-jun and c-fos induced by Npe6-mediated PDT Were inhibited by SB203580, p38 MAPK inhibitors, and the expression of VEGF mRNA was inhibited by cycloheximide (CHX), a protein synthesis inhibitor. The c-jun mRNA expression was inhibited, whereas the c-fos mRNA expression was enhanced by N-acetyl-L-cysteine (NAC), a free radical scavenger. We conclude that Npe6-mediated PDT induces the expression of VEGF, c-jun and c-fos in human oral carcinoma cell line, HSC-3 cell, and at least partly, through the activation of p38 MAPK. (c) 2006 Published by Elsevier Ltd.