Staufen- and FMRP-containing neuronal RNPs are structurally and functionally related to somatic P bodies
Staufen- and FMRP-containing neuronal RNPs are structurally and functionally related to somatic P bodies
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DOI：
10.1016/j.neuron.2006.10.028
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发表时间：
2006-12-21
期刊：
影响因子：
16.2
通讯作者：
Ramaswami, Mani
中科院分区：
文献类型：
--
作者：
Barbee, Scott A.;Estes, Patricia S.;Ramaswami, Mani
mRNA翻译的局部控制调节神经元发育、突触可塑性和记忆形成。这种控制的一个鲜为人知的方面是介导神经元rna转运和翻译的核糖核蛋白（RNP）颗粒的作用和组成。在这里，我们发现果蝇神经元中含有staufen-和fmrp的RNPs也含有体细胞“P体”中存在的蛋白质，包括rna降解酶Dcp1p和Xrn1p/Pacman以及miRNA （argonaute）、NMD （Upf1p）和一般翻译抑制（Dhh1p/Me31B）途径的关键成分。果蝇Me31B被证明参与(1)与fmrp相关的P体蛋白（Scd6p/trailer hitch）参与fmrp驱动的、argonaute依赖的眼成像盘发育中的翻译抑制；(2)幼虫感觉神经元的树突发育；(3)小矮脚猴mirna介导的翅膀成像盘的翻译抑制。这些结果证明了对神经元功能至关重要的翻译控制的保守机制，并为理解神经元内mRNA功能的调节开辟了新的实验途径。
Local control of mRNA translation modulates neuronal development, synaptic plasticity, and memory formation. A poorly understood aspect of this control is the role and composition of ribonucleoprotein (RNP) particles that mediate transport and translation of neuronal RNAs. Here, we show that staufen- and FMRP-containing RNPs in Drosophila neurons contain proteins also present in somatic "P bodies," including the RNA-degradative enzymes Dcp1p and Xrn1p/Pacman and crucial components of miRNA (argonaute), NMD (Upf1p), and general translational repression (Dhh1p/Me31B) pathways. Drosophila Me31B is shown to participate (1) with an FMRP-associated, P body protein (Scd6p/trailer hitch) in FMRP-driven, argonaute-dependent translational repression in developing eye imaginal discs; (2) in dendritic elaboration of larval sensory neurons; and (3) in bantam miRNA-mediated translational repression in wing imaginal discs. These results argue for a conserved mechanism of translational control critical to neuronal function and open up new experimental avenues for understanding the regulation of mRNA function within neurons.