Vitamin a storage in hepatic stellate cells in the regenerating rat liver: With special reference to zonal heterogeneity
Vitamin a storage in hepatic stellate cells in the regenerating rat liver: With special reference to zonal heterogeneity
复制标题
DOI：
10.1002/ar.a.20230
复制
发表时间：
2005-10-01
期刊：
影响因子：
--
通讯作者：
Senoo, H
中科院分区：
文献类型：
--
作者：
Higashi, N;Sato, M;Senoo, H
在生理条件下，肝小叶内的肝星状细胞（HSC）将约80%的全身维生素A储存在其细胞质中的脂滴中，并且这些细胞在维生素A储存能力方面显示出区域异质性。维生素A对于细胞的生长和分化是必需的，并且众所周知，包括HSC在内的肝细胞在部分肝切除术（PHx）后显示出显著的生长能力。然而，维生素A在肝再生中在HSC中的储存状态尚不清楚。因此，我们进行了本研究，以检查在肝再生过程中这些细胞中的维生素A储存。在电子显微镜水平上的形态测量，维生素A自发荧光的荧光显微镜，结蛋白和α-平滑肌肌动蛋白（α-SMA）的免疫荧光显微镜下进行的雄性Wistar品系大鼠的肝脏切片在不同的时间后，动物已受到70%PHx。PHx后3d，每个HSC储存维生素A的脂滴面积逐渐减少，14 d后恢复正常，但肝小叶内每个HSC储存维生素A的脂滴面积的异质性在PHx后消失，14 d后仍未恢复正常，尽管肝脏体积已恢复正常。这些结果表明，HSC在肝再生过程中改变其维生素A储存能力，恢复维生素A稳态需要比肝脏体积长得多的时间。(C)2005 Wiley-Liss，Inc.
Under physiological conditions, hepatic stellate cells (HSCs) within liver lobules store about 80% of the total body vitamin A in lipid droplets in their cytoplasm, and these cells show zonal heterogeneity in terms of vitamin A-storing capacity. Vitamin A is essential for the growth and differentiation of cells, and it is well known that liver cells including HSCs show a remarkable growth capacity after partial hepatectomy (PHx). However, the status of vitamin A storage in HSCs in the liver regeneration is not yet known. Therefore, we conducted the present study to examine vitamin A storage in these cells during liver regeneration. Morphometry at the electron microscopic level, fluorescence microscopy for vitamin A autofluorescence, and immunofluorescence microscopy for desmin and alpha-smooth muscle actin (alpha-SMA) were performed on sections of liver from male Wistar strain rats at various times after the animal had been subjected to 70% PHx. The mean area of vitamin A-storing lipid droplets per HSC gradually decreased toward 3 days after PHx, and then returned to normal within 14 days after it. However, the heterogeneity of vitamin A-storing lipid droplet area per HSC within the hepatic lobule disappeared after PHx and did not return to normal by 14 days thereafter, even though the liver volume had returned to normal. These results suggest that HSCs alter their vitamin A-storing capacity during liver regeneration and that the recovery of vitamin A homeostasis requires a much longer time than that for liver volume. (C) 2005 Wiley-Liss, Inc.