Construction, expression and functional analysis of a glycolipid‐linked form of CR1
Construction, expression and functional analysis of a glycolipid‐linked form of CR1
复制标题
CR1 糖脂连接形式的构建、表达和功能分析
DOI：
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复制
发表时间：
1993
影响因子：
5.4
通讯作者：
P. Lachmann
中科院分区：
文献类型：
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作者：
P. Clissold;H. Ebling;P. Lachmann
By genetic engineering of human CR1 cDNA and its stable transfection into cells we have produced a cell line which expresses CR1 anchored to the cell surface by a glycolipid anchor. The glycosyl‐phosphatidylinositol (GPI)‐CRl protects cells intrinsically from damage mediated by complement activated through the classical pathway. Cell surface GPI‐CR1 is more efficient on a molar basis than soluble CR1 in the assay, but extrinsic protection of other cells was not obtained. Soluble CRl‐protected cells extrinsically in the assay but was required at nearly ten fold higher amounts than the intrinsic protection conferred by GPI‐anchored CR1. Additionally, GPI‐CR1 was shown to act as a co‐factor to Factor I in the generation of C3c from iC3b. Since GPI‐anchored proteins can incorporate spontaneously into the membranes of living cells by virtue of their lipid tails, the isolated GPI‐CR1 will be used to introduce CR1 on to the surfaces of many different types of cell so that its role in immunity can be further investigated.
可溶性 CR1 在补体和中性粒细胞介导的组织损伤中的保护作用。
DOI：
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发表时间：
1992
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Mulligan,MS;Yeh,CG;Rudolph,AR;Ward,PA
通讯作者：
Ward,PA
衰变加速因子作为人类 T 细胞的信号转导分子。
DOI：
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发表时间：
1988
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Davis,LS;Patel,SS;Atkinson,JP;Lipsky,PE
通讯作者：
Lipsky,PE
影响因子：
4.4
作者：
Joseph A. Hill;THOMAS F. Lindsay;F. Ortiz;C. Yeh;Herbert B. Hechtman;Francis D. Moore
通讯作者：
Joseph A. Hill;THOMAS F. Lindsay;F. Ortiz;C. Yeh;Herbert B. Hechtman;Francis D. Moore