PROPERTIES OF A HEPARIN-POLY(VINYL ALCOHOL) HYDROGEL COATING
PROPERTIES OF A HEPARIN-POLY(VINYL ALCOHOL) HYDROGEL COATING
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DOI：
10.1002/jbm.820170212
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
SEFTON, MV
中科院分区：
文献类型：
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作者：
GOOSEN, MFA;SEFTON, MV
肝素-PVA [聚乙烯醇]水凝胶的一些物理、化学和生物学特性被确定，该水凝胶可能用作制备小直径人造血管和采血导管的抗血栓涂层。缩醛交联凝胶中交联键之间的MW为约8000，允许白蛋白大小的蛋白质进入凝胶内部。肝素从含有7 mg/g凝胶的凝胶中的释放速率为10-2 μ g/g min，这显著低于所报道的离子肝素化材料的抗血栓性所需的最小值。然而，在凝血酶时间和血浆凝固时间测定中均观察到体外生物活性，这不能归因于肝素释放到孵育血浆中。3份肝素样品中最终肝素含量与末端氨基酸残基量的相关性表明，肝素通过氨基酸末端与凝胶中的PVA结合;这为固定化肝素保留生物活性提供了合理的解释。
Some physical, chemical and biological characteristics of a heparin-PVA[poly(vinyl alcohol)] hydrogel potentially useful as a thromboresistant coating for the preparation of small-diameter vascular prostheses and blood-sampling catheters were determined. The MW between crosslinks in the acetal crosslinked gel was .apprx. 8000, permitting proteins the size of albumin to enter the interior of the gel. The release rate of heparin from a gel containing 7 mg/g gel was 10-2 .mu.g/g min which was significantly lower than the reported minimum required for thromboresistance of ionically heparinized materials. Nevertheless, in vitro biological activity was observed in both thrombin time and plasma recalcification time assays, which could not be attributed to the release of heparin into the incubated plasma. Correlation of final heparin contents with the amount of terminal amino acid residues in 3 samples of heparin suggests that the heparin is bound to the PVA in the gel through the amino acid terminus; this provides a plausible explanation for the retention of biological activity by the immobilized heparin.