Bioluminescence imaging of lymphocyte trafficking in vivo
Bioluminescence imaging of lymphocyte trafficking in vivo
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DOI：
10.1016/s0301-472x(01)00756-1
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发表时间：
2001-12-01
影响因子：
2.6
通讯作者：
Contag, CH
中科院分区：
文献类型：
--
作者：
Hardy, J;Edinger, M;Contag, CH
淋巴细胞是高度移动的细胞，响应于各种各样的刺激而在全身旅行。揭示淋巴细胞在体内的运输模式是必要的免疫功能，以及在免疫发育和响应侮辱的细胞-细胞和细胞-组织的相互作用的完整理解。尽管可以通过诸如流式细胞术和免疫荧光的技术来揭示在任何给定时间点细胞群在各种组织中的位置，但是这些方法不容易适用于体内动态细胞迁移模式的评估。在过去的5年中，成像活体动物的分子和细胞变化的技术已经发展到可以揭示这些至关重要的细胞的迁移路径的程度。在这里，我们回顾了细胞成像的一个进展，在体内生物发光成像，它解决了淋巴细胞跟踪的问题。这种成像策略有可能阐明免疫反应的时间模式和体内淋巴细胞的空间分布。(C)2001年国际实验血液学学会。出版社：Elsevier Science Inc.
Lymphocytes are highly mobile cells that travel throughout the body in response to a tremendous variety of stimuli. Revealing lymphocyte trafficking patterns in vivo is necessary for a complete understanding of immune function, as well as cell-cell and cell-tissue interactions in immune development and in response to insult. Although the location of cell populations in various tissues at any given point in time may be revealed by techniques such as flow cytometry and immunofluorescence, these methods are not readily amenable to the assessment of dynamic cell migration patterns in vivo. In the past 5 years, technologies for imaging molecular and cellular changes in living animals have advanced to a point where it is possible to reveal the migratory paths of these vitally important cells. Here, we review one advancement in cellular imaging, in vivo bioluminescence imaging, which addresses the problem of lymphocyte tracking. This imaging strategy has the potential to elucidate the temporal patterns of immune responses and the spatial distribution of lymphocytes within the body. (C) 2001 International Society for Experimental Hematology. Published by Elsevier Science Inc.