Interleukin-18 enhances lipopolysaccharide-induced interferon-γ production in human whole blood cultures
Interleukin-18 enhances lipopolysaccharide-induced interferon-γ production in human whole blood cultures
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DOI：
10.1086/314481
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发表时间：
1998-12-01
影响因子：
6.4
通讯作者：
Dinarello, CA
中科院分区：
文献类型：
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作者：
Puren, AJ;Razeghi, P;Dinarello, CA
白细胞介素-18（IL-18）是一种新发现的细胞因子，以前称为干扰素-γ（IFN-γ）诱导因子。在简单的24小时人全血培养中，IFN-γ由脂多糖（LPS）加IL-18的组合产生。为了释放白细胞和红细胞区室中的细胞因子，将去污剂Triton X-100加入到整个血液培养物中。低浓度的LPS加IL-18的组合诱导的IFN-γ的产生比单独的任一刺激物高3至5倍。还产生肿瘤坏死因子-α（TNF-α）、IL-6和IL-8。IL-10的存在完全抑制了IFN-γ的产生，并减少了TNF-α、IL-6和IL-8的产生。因此，IFN-γ、TNF-α、IL-8和IL-6在单一全血培养物中产生，使得在单一培养物中1型T辅助细胞因子和促炎细胞因子的合成与疾病活性的相关性成为可能。
Interleukin-18 (IL-18) is a newly described cytokine, formerly called interferon-gamma (IFN-gamma)-inducing factor. Tn a simple 24-h human whole blood culture, IFN-gamma was produced by the combination of lipopolysaccharide (LPS) plus IL-18. To liberate cytokines in the leukocyte and red cell compartments, the detergent Triton X-100 was added to the entire blood culture. The combination of low concentrations of LPS plus IL-18 induced a 3- to 5-fold greater production of IFN-gamma than did either stimulant alone. Tumor necrosis factor-alpha (TNF-alpha), IL-6, and IL-8 were also produced. The presence of IL-10 completely suppressed the production of IFN-gamma and reduced that of TNF-alpha, IL-6, and IL-8, Thus, IFN-gamma, TNF-alpha, IL-8, and IL-6 are produced in a single whole blood culture, making correlations in the synthesis of a T helper type 1 cytokine and proinflammatory cytokines with disease activity possible in a single culture.