Waveguide interrogated optical immunosensor (WIOS) for detection of sulfonamide antibiotics in milk
Waveguide interrogated optical immunosensor (WIOS) for detection of sulfonamide antibiotics in milk
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DOI：
10.1016/j.bios.2009.04.036
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发表时间：
2009-07-15
影响因子：
12.6
通讯作者：
Voirin, Guy
中科院分区：
文献类型：
--
作者：
Adrian, Javier;Pasche, Stephanie;Voirin, Guy
研制了一种检测牛奶中磺胺类抗生素的免疫传感器。检测依赖于竞争性免疫测定格式，使用以前开发的磺胺类抗生素的通用检测和酶联免疫吸附测定评价的免疫试剂。该免疫测定在微系统平台上实施，波长询问光学传感装置，其使用渐逝场探测波导层界面处的变化，从而允许灵敏地检测生物分子吸附。将免疫试剂固定到波导芯片的表面上，并且流体单元允许分析物和检测溶液在表面上方流动。磺胺吡啶用作参比磺胺，在缓冲液和牛奶中用免疫传感器检测，检测限（IC（90））分别为0.2 +/- 0.1 μ g L（-1）和0.5 +/- 0.1 μ g L（-1）。分析时间低于30分钟，包括传感表面的再生，所需的样品制备最少。检测的重复性好于10%。一个盲法检测允许识别牛奶样品被不同的磺胺类抗生素污染，达到或超过欧盟为这些化合物建立的最大残留限量（100 μ g L（-1））。因此，所开发的免疫传感器作为通用传感装置，为非熟练用户在现场快速和早期检测食品污染物提供了巨大的潜力。(C)2009 Elsevier B. V.保留所有权利。
An immunosensor was developed for the detection of sulfonamide antibiotics in milk. Detection relied on a competitive immunoassay format, using immunoreagents previously developed for the generic detection of sulfonamide antibiotics and evaluated by enzyme-linked immunosorbent assay. The immunoassay was implemented onto a microsystem platform, the wavelength interrogated optical sensing device, which uses the evanescent field to probe changes at the interface of a waveguiding layer, and thus allows sensitive detection of biomolecule adsorption. The immunoreagents were immobilized onto the surface of the waveguide chip, and a fluidic cell allowed flowing analyte and detection solutions above the surface. Sulfapyridine was used as reference sulfonamide and detected with the immunosensor in buffer and ;in milk with a limit of detection (IC(90)) of 0.2 +/- 0.1 mu g L(-1) and 0.5 +/- 0.1 mu g L(-1), respectively. The analysis time was below 30 min, including regeneration of the sensing surface, with minimum sample preparation required. The reproducibility of the detection was better than 10%. A blind assay allowed identifying milk samples that were contaminated with different sulfonamide antibiotics at or above the maximum residue limits established by the European Union for these compounds (100 mu g L(-1)). Thus, the developed immunosensor presents great potential as a generic sensing device for the fast and early detection of food contaminants on the field by non-skilled users. (C) 2009 Elsevier B.V. All rights reserved.