The global carbon metabolism regulator Crc is a component of a signal transduction pathway required for biofilm development by Pseudomonas aeruginosa
The global carbon metabolism regulator Crc is a component of a signal transduction pathway required for biofilm development by Pseudomonas aeruginosa
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DOI：
10.1128/jb.182.2.425-431.2000
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发表时间：
2000-01-01
影响因子：
3.2
通讯作者：
Kolter, R
中科院分区：
文献类型：
--
作者：
O'Toole, GA;Gibbs, KA;Kolter, R
从浮游（自由游动）存在到附着在生物膜表面的生长的转变是响应于环境因素，包括营养物质的可用性。我们表明，分解代谢产物阻遏控制（Crc）蛋白，它在碳代谢的调节中发挥作用，是必要的铜绿假单胞菌生物膜的形成，使用相差显微镜，我们发现，一个cre突变体只使一个分散的单层细胞在塑料表面上，但不开发密集的单层由小菌落典型的野生型菌株。这是一个表型相同的突变体中观察到的缺陷在IV型菌毛生物发生，与此观察一致，cre突变体是缺陷的IV型菌毛介导的抽搐运动，我们表明，这种缺陷在IV型菌毛功能是由于（至少部分）减少在pill（菌毛蛋白）转录。我们建议，营养线索是由Crc作为调节生物膜发育的信号转导途径的一部分整合的。
The transition from a planktonic (free-swimming) existence to growth attached to a surface in a biofilm occurs in response to environmental factors, including the availability of nutrients. We show that the catabolite repression control (Crc) protein, which plays a role in the regulation of carbon metabolism, is necessary for biofilm formation in Pseudomonas aeruginosa, Using phase-contrast microscopy, we found that a cre mutant only makes a dispersed monolayer of cells on a plastic surface but does not develop the dense monolayer punctuated by microcolonies typical of the wild-type strain. This is a phenotype identical to that observed in mutants defective in type TV pilus biogenesis, Consistent with this observation, cre mutants are defective in type IV pilus-mediated twitching motility, We show that this defect in type IV pilus function is due (at least in part) to a decrease in pill (pilin) transcription. We propose that nutritional cues are integrated by Crc as part of a signal transduction pathway that regulates biofilm development.