Effect of experimentally induced hypothyroidism on sulfate renal transport in rats.
Effect of experimentally induced hypothyroidism on sulfate renal transport in rats.
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实验诱导的甲状腺功能减退症对大鼠硫酸盐肾转运的影响。
DOI：
10.1152/ajprenal.1999.276.1.f164
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Morris,ME
中科院分区：
文献类型：
--
作者：
Sagawa,K;Murer,H;Morris,ME
Decreased serum sulfate concentrations are observed in hypothyroid patients. However, the mechanism involved in thyroid hormone-induced alterations of renal sulfate homeostasis is unknown. The objectives of this investigation were to determine the effect of 6-propyl-2-thiouracil (PTU)-induced hypothyroidism in rats on1) the in vivo serum concentrations, renal clearance, and renal reabsorption of sulfate,2) the in vitro renal transport in brush-border membrane (BBM) and basolateral membrane (BLM) vesicles, and3) the cellular mechanism of the hypothyroid-induced alteration in sulfate renal transport. Serum sulfate concentrations, renal fractional reabsorption of sulfate, and creatinine clearance were decreased significantly in the hypothyroid group. TheVmaxvalues for sodium-sulfate cotransport in BBM were significantly decreased in the kidney cortex from the hypothyroid animals (0.90 ± 0.31 vs. 0.49 ± 0.08 nmol ⋅ mg−1⋅ 10 s−1,n= 5–6,P< 0.05) without changes inKm. There were no significant differences inVmaxandKmfor sulfate/anion exchange transport in BLM. Sodium-dependent sulfate transporter (NaSi-1) mRNA and protein levels were significantly lower in the kidney cortex from hypothyroid rats. Hypothyroidism did not alter the membrane motional order (fluidity) in BBM and BLM, which indicates that the changes in the membrane fluidity do not represent the mechanism for the altered renal transport. These results demonstrate that PTU-induced hypothyroidism decreases sodium-sulfate cotransport by downregulation of the NaSi-1 gene.
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DOI：
--
发表时间：
1995
期刊：
The Journal of pharmacology and experimental therapeutics.
影响因子：
--
作者：
Benincosa,LJ;Sagawa,K;Morris,ME
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Morris,ME
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DOI：
--
发表时间：
1993
期刊：
Drug metabolism and disposition: the biological fate of chemicals
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作者：
Benincosa,LJ;Morris,ME
通讯作者：
Morris,ME
放射性硫酸盐测定人和狗细胞外液体积的评估。
DOI：
10.1172/jci102739
发表时间：
1953
期刊：
The Journal of clinical investigation
影响因子：
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作者：
M. Walser;D. Seldin;A. Grollman
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A. Grollman
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DOI：
10.1152/ajprenal.1984.246.2.f133
发表时间：
1984
期刊：
The American journal of physiology
影响因子：
--
作者：
Espinosa,RE;Keller,MJ;Yusufi,AN;Dousa,TP
通讯作者：
Dousa,TP
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影响因子：
4.8
作者：
Yusufi,AN;Murayama,N;Keller,MJ;Dousa,TP
通讯作者：
Dousa,TP