Use of low-angle diamond knives leads to improved ultrastructural preservation of ultrathin sections.
Use of low-angle diamond knives leads to improved ultrastructural preservation of ultrathin sections.
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使用低角度金刚石刀可以改善超薄切片的超微结构保存。
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
J. Jésior
中科院分区：
文献类型：
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作者：
J. Jésior
低角度金刚石刀，其中一个角度低至14.6度，已被测试用于测量嵌入环氧树脂的聚苯乙烯乳胶球切割过程中产生的压缩。压缩减少大致成比例的切割角度（刀和间隙角的总和）。低角度金刚石刀在生物和材料科学中的有益应用，通过肌肉纤维的切片（压缩减少了3倍），胶原纤维的切片（子午分辨率提高了2倍）和铜栅条的切片（减少卷曲）来说明。这些低角度的钻石刀与经典的刀有45度的角度一样耐药，作者经常使用。低角度的金刚石刀，角度低至35度，现在可以在市场上买到。
Low-angle diamond knives, one of them with an angle as low as 14.6 degrees, have been tested to measure compression induced during the sectioning of polystyrene latex spheres embedded in epoxy resins. Compression diminishes roughly proportional to the sectioning angle (sum of knife and clearance angles). The beneficial application of low-angle diamond knives in biology and material science is illustrated by sections through a muscle fiber (compression reduced by a factor of 3), sections through a collagen fiber (meridional resolution improved by a factor of 2) and sections through a copper grid bar (reduced curling). These low-angle diamond knives are as resistant as the classical knives having angles of 45 degrees and are routinely used by the author. Low-angle diamond knives with angles down to 35 degrees are now commercially available.