Nucleotide sugar transporters: elucidation of their molecular identity and its implication for future studies.
Nucleotide sugar transporters: elucidation of their molecular identity and its implication for future studies.
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核苷酸糖转运蛋白：阐明其分子特性及其对未来研究的意义。
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
S. Yoshioka
中科院分区：
文献类型：
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作者：
M. Kawakita;N. Ishida;N. Miura;G. Sun;S. Yoshioka
核苷酸糖转运蛋白主要位于高尔基体膜中，并将高尔基体外部产生的核苷酸糖携带到细胞器中，在那里它们作为糖基转移酶延长碳水化合物链的底物。因此，它们对于细胞糖复合物的合成是不可或缺的，此外，它们可能在产生细胞糖复合物的结构多样性方面具有调节作用。它们的存在早已得到充分认识，但由于缺乏其精确分子结构的信息，对其严格底物特异性和作用模式的分子基础的研究受到阻碍。最近克隆了编码其中几种转运蛋白的互补 DNA，这代表着在上述问题方面向前迈出了实质性的一步。这些cDNA的产物是相互相关的疏水蛋白，由320-400个氨基酸残基组成，具有多个假定的跨膜螺旋结构域，并且位于高尔基体中。本文简要总结了核苷酸糖转运蛋白领域的研究现状，并对该领域的研究进行了展望。
Nucleotide sugar transporters are mainly located in the Golgi membranes and carry nucleotide sugars, that are produced outside the Golgi apparatus, into the organelle, where they serve as substrates for the elongation of carbohydrate chains by glycosyltransferases. They are thus indispensable for cellular glycoconjugate synthesis and, moreover, may have regulatory roles in producing the structural variety of cellular glycoconjugates. Their occurrence has long been well recognized, but studies on the molecular bases of their strict substrate specificities and modes of action have been hampered by the lack of information on their precise molecular structures. Complementary DNAs encoding several of these transporters were cloned recently, which represented a substantial step forward as to the above mentioned issues. The products of these cDNAs are mutually related hydrophobic proteins consisting of 320-400 amino acid residues with multiple putative transmembrane helix domains, and are located in the Golgi apparatus. This review briefly summarizes the present status of the field of nucleotide sugar transporter research, and also presents an outlook of the study in this field.