Effective methods for the inactivation of Francisella tularensis
Effective methods for the inactivation of Francisella tularensis
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DOI：
10.1371/journal.pone.0225177
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发表时间：
2019-11-14
期刊：
影响因子：
3.7
通讯作者：
Morikawa, Shigeru
中科院分区：
文献类型：
--
作者：
Azaki, Mika;Uda, Akihiko;Morikawa, Shigeru
土拉弗朗西斯菌（Francisella tularensis）F.土拉热）对人类具有高致病性，必须在生物安全三级条件下处理。用于诊断和实验分析的样品必须完全灭活，尽管灭活F.土拉热是有限的。本研究探讨了灭活F.土拉热菌SCHUP 9和五种其它菌株通过比较处理样品和对照样品之间的菌落形成单位来确定。结果表明，F.通过热处理（94 ℃ 3分钟和56 ℃ 30分钟）、用0.22 μ m过滤器过滤和使用各种溶液（即> 70%乙醇、甲醇、丙酮和4%多聚甲醛）使土拉热菌SCHUP 9变性。F.在用50%乙醇处理1分钟，用0.45 μ m过滤器过滤，并用去污剂（即1%十二烷基硫酸锂缓冲液，1%Triton X-100和1%Nonidet P-40）在4 ℃处理24小时后，土拉热菌SCHUP 9保持存活。此外，F.与在水和化学成分确定的培养基中的悬浮液相比，在塑料管中悬浮在胎牛血清中的土拉热菌SCHUP 9对紫外线辐射具有高度抗性。对F.土拉热菌SCHUP 9对其他5株F.土拉热。本研究中的数据可用于建立指南和标准操作程序（SOP），以使受污染的样品不仅在F。土拉菌，还有其他细菌。
Francisella tularensis (F. tularensis) is highly pathogenic to humans and must be handled under biosafety level 3 conditions. Samples used for the diagnosis and experimental analysis must be completely inactivated, although methods for the inactivation of F. tularensis are limited. In this study, effective methods for the inactivation of F. tularensis SCHU P9 and five other strains were determined by comparisons of colony-forming units between treated and control samples. The results showed that F. tularensis SCHU P9 was denatured by heat treatment (94 degrees C for 3 min and 56 degrees C for 30 min), filtration with a 0.22 mu m filter, and the use of various solutions (i.e. > 70% ethanol, methanol, acetone, and 4% paraformaldehyde). F. tularensis SCHU P9 remained viable after treatment with 50% ethanol for 1 min, filtration with a 0.45 mu m filter, and treatments with detergents (i.e. 1% lithium dodecyl sulfate buffer, 1% Triton X-100 and 1% Nonidet P-40) at 4 degrees C for 24 h. Additionally, F. tularensis SCHU P9 suspended in fetal bovine serum in plastic tubes was highly resistant to ultraviolet radiation compared to suspensions in water and chemically defined medium. The methods for inactivation of F. tularensis SCHU P9 was applicable to the other five strains of F. tularensis. The data presented in this study could be useful for the establishment of guidelines and standard operating procedures (SOP) to inactivate the contaminated samples in not only F. tularensis but also other bacteria.