THE EFFECT OF ACONITINE ON THE GIANT AXON OF THE SQUID.
THE EFFECT OF ACONITINE ON THE GIANT AXON OF THE SQUID.
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DOI：
10.1085/jgp.47.4.719
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发表时间：
1964-03
影响因子：
3.8
通讯作者：
BRYANT, S H
中科院分区：
文献类型：
--
作者：
HERZOG, W H;FEIBEL, R M;BRYANT, S H
在Loligo pealii的巨大轴突中，“乌头碱强效”默克加入了浴缸（10-7至1.25 × 10-6 gm/ml）（a）对静息膜电位、膜电阻和整流、膜对阈下电流的反应、临界去极化或动作电位没有影响，但（B）对重复刺激产生尖峰后的膜电位振荡，去极化，膜电阻降低。这种效应与连续的动作电位相加;它随乌头碱浓度、暴露时间和刺激频率而增加。当振荡足够大，膜电位为51.6 ± SD 1.5 mV时，一个自我维持的活动开始爆发;它通常持续20到70秒。膜电位为41.5 ± SD 1.9mv。复极化发生的时间常数为2.5至11.1分钟。胆碱替代外部钠后，突发超极化膜至-70毫伏，并返回到正常的外部钠去极化再次超过静息膜电位。乌头碱对细胞膜的作用是由于动作电位后钠、钾或氯离子电导的增加。
In the giant axon of Loligo pealii, "aconitine potent" Merck added to the bath (10-7 to 1.25 x 10-6 gm/ml) (a) had no effect on resting membrane potential, membrane resistance and rectification, membrane response to subthreshold currents, critical depolarization, or action potential, but (b) on repetitive stimulation produced oscillations of membrane potential after the spike, depolarization, and decrease of membrane resistance. The effect sums with successive action potentials; it increases with concentration of aconitine, time of exposure, and frequency of stimulation. When the oscillations are large enough and the membrane potential is 51.6 ± SD 1.5 mv a burst of self-sustained activity begins; it usually lasts 20 to 70 sec. and at its end the membrane potential is 41.5 ± SD 1.9 mv. Repolarization occurs with a time constant of 2.5 to 11.1 min. Substitution of choline for external sodium after a burst hyperpolarizes the membrane to -70 mv, and return to normal external sodium depolarizes again beyond the resting membrane potential. The effect of aconitine on the membrane is attributed to an increase of sodium and potassium or chloride conductances following the action potential.